Increased nonenzymatically glycosylated proteins in the vitreous humor of diabetic animals.

Published

Journal Article

One of the earliest pathologic changes of diabetes mellitus is increased nonenzymatic glycosylation (i.e., glycation) of proteins, which results in abnormal aggregation of collagen fibrils and production of superoxide radicals. These abnormalities may be responsible for the precocious senescence of connective tissue associated with the disease. We sought to determine whether glycation is increased in the vitreous humor of short-term diabetic cats (6 months' duration) and rabbits (2 months' duration), using a nitroblue tetrazolium colorimetric assay for fructosamine. Vitreous protein fructosamine concentration was significantly higher in diabetic cats and rabbits, compared with that in control (nondiabetic) animals. These results indicate that glycation is increased in the vitreous humor of short-term diabetic animals, and therefore may be one of the initial triggers for clinically apparent diabetic retinopathy.

Full Text

Duke Authors

Cited Authors

  • Farr, AK; Braun, RD; Cefalu, WT; Bell-Farrow, AD; Wang, ZQ; Hatchell, DL

Published Date

  • February 1999

Published In

Volume / Issue

  • 49 / 1

Start / End Page

  • 58 - 61

PubMed ID

  • 10090096

Pubmed Central ID

  • 10090096

International Standard Serial Number (ISSN)

  • 0023-6764

Language

  • eng