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Recent advances for measurement of protein synthesis rates, use of the 'Virtual Biopsy' approach, and measurement of muscle mass.

Publication ,  Journal Article
Hellerstein, M; Evans, W
Published in: Curr Opin Clin Nutr Metab Care
May 2017

PURPOSE OF REVIEW: Flux-rate measurements of protein synthesis and breakdown (turnover) in muscle represent an ideal class of mechanism-based biomarkers for conditions of altered muscle mass and function. We describe here new metabolic labeling techniques for flux-rate measurements in humans, focusing on skeletal muscle. RECENT FINDINGS: Dynamics of the muscle proteome are accurately measured in humans by combining long-term heavy water labeling with tandem mass spectrometry. Broad proteomic flux signatures or kinetics of targeted proteins are measurable. After interventions, early fractional synthesis rates of skeletal muscle proteins predict later changes in muscle mass. The 'virtual biopsy' method for measuring tissue protein turnover rates from body fluids has been validated for skeletal muscle, from labeling of plasma creatine kinase-type M or carbonic anhydrase-3. Label in these proteins in plasma reflects label of cognate proteins in the tissue, and response in plasma predicts longer term outcomes. Skeletal muscle mass can also be measured noninvasively from a spot urine, based on dilution of labeled creatine. This method correlates well with whole body MRI assessment of muscle mass and predicts clinical outcomes in older men. SUMMARY: Flux measurements are available and more interpretable functionally than static measurements for several reasons, which are discussed.

Duke Scholars

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Published In

Curr Opin Clin Nutr Metab Care

DOI

EISSN

1473-6519

Publication Date

May 2017

Volume

20

Issue

3

Start / End Page

191 / 200

Location

England

Related Subject Headings

  • Tandem Mass Spectrometry
  • Staining and Labeling
  • Proteomics
  • Proteome
  • Protein Biosynthesis
  • Nutrition & Dietetics
  • Muscular Diseases
  • Muscle, Skeletal
  • Muscle Proteins
  • Humans
 

Citation

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ICMJE
MLA
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Hellerstein, M., & Evans, W. (2017). Recent advances for measurement of protein synthesis rates, use of the 'Virtual Biopsy' approach, and measurement of muscle mass. Curr Opin Clin Nutr Metab Care, 20(3), 191–200. https://doi.org/10.1097/MCO.0000000000000370
Hellerstein, Marc, and William Evans. “Recent advances for measurement of protein synthesis rates, use of the 'Virtual Biopsy' approach, and measurement of muscle mass.Curr Opin Clin Nutr Metab Care 20, no. 3 (May 2017): 191–200. https://doi.org/10.1097/MCO.0000000000000370.
Hellerstein, Marc, and William Evans. “Recent advances for measurement of protein synthesis rates, use of the 'Virtual Biopsy' approach, and measurement of muscle mass.Curr Opin Clin Nutr Metab Care, vol. 20, no. 3, May 2017, pp. 191–200. Pubmed, doi:10.1097/MCO.0000000000000370.

Published In

Curr Opin Clin Nutr Metab Care

DOI

EISSN

1473-6519

Publication Date

May 2017

Volume

20

Issue

3

Start / End Page

191 / 200

Location

England

Related Subject Headings

  • Tandem Mass Spectrometry
  • Staining and Labeling
  • Proteomics
  • Proteome
  • Protein Biosynthesis
  • Nutrition & Dietetics
  • Muscular Diseases
  • Muscle, Skeletal
  • Muscle Proteins
  • Humans