The effect of osmotic stress on the production of nukacin ISK-1 from Staphylococcus warneri ISK-1.

Journal Article (Journal Article)

The effects of several additives on the production of a lantibiotic, nukacin ISK-1, from Staphylococcus warneri ISK-1, in batch fermentation were studied. NaCl, KCl and sorbitol stimulated nukacin ISK-1 production. The addition of 1.4 M NaCl increased nukacin ISK-1 activity 1.5-fold over the control, while cell growth and glucose consumption were inhibited. Nukacin ISK-1 production increased with increasing osmolarity of the medium up to about 3 osmol/kg; however, further increases in osmolarity diminished productivity, irrespective of the kind of additive. Northern blot analysis showed that transcription of the nukacin ISK-1 structural gene (nukA) was activated in the presence of 1.4 M NaC1. These data indicate that the stimulation effect was due to osmotic stress, which acted, at least in part, at the transcriptional level on the nukA gene.

Full Text

Duke Authors

Cited Authors

  • Sashihara, T; Dan, M; Kimura, H; Matsusaki, H; Sonomoto, K; Ishizaki, A

Published Date

  • August 2001

Published In

Volume / Issue

  • 56 / 3-4

Start / End Page

  • 496 - 501

PubMed ID

  • 11549026

International Standard Serial Number (ISSN)

  • 0175-7598

Digital Object Identifier (DOI)

  • 10.1007/s002530100669


  • eng

Conference Location

  • Germany