Expanded Satellite Repeats Amplify a Discrete CENP-A Nucleosome Assembly Site on Chromosomes that Drive in Female Meiosis.

Published

Journal Article

Female meiosis provides an opportunity for selfish genetic elements to violate Mendel's law of segregation by increasing the chance of segregating to the egg [1]. Centromeres and other repetitive sequences can drive in meiosis by cheating the segregation process [2], but the underlying mechanisms are unknown. Here, we show that centromeres with more satellite repeats house more nucleosomes that confer centromere identity, containing the histone H3 variant CENP-A, and bias their segregation to the egg relative to centromeres with fewer repeats. CENP-A nucleosomes predominantly occupy a single site within the repeating unit that becomes limiting for centromere assembly on smaller centromeres. We propose that amplified repetitive sequences act as selfish elements by promoting expansion of CENP-A chromatin and increased transmission through the female germline.

Full Text

Duke Authors

Cited Authors

  • Iwata-Otsubo, A; Dawicki-McKenna, JM; Akera, T; Falk, SJ; Chmátal, L; Yang, K; Sullivan, BA; Schultz, RM; Lampson, MA; Black, BE

Published Date

  • August 7, 2017

Published In

Volume / Issue

  • 27 / 15

Start / End Page

  • 2365 - 2373.e8

PubMed ID

  • 28756949

Pubmed Central ID

  • 28756949

Electronic International Standard Serial Number (EISSN)

  • 1879-0445

Digital Object Identifier (DOI)

  • 10.1016/j.cub.2017.06.069

Language

  • eng

Conference Location

  • England