The effect of Bacopa monnieri on gene expression levels in SH-SY5Y human neuroblastoma cells.

Journal Article (Journal Article)

Bacopa monnieri is a plant used as a nootropic in Ayurveda, a 5000-year-old system of traditional Indian medicine. Although both animal and clinical studies supported its role as a memory enhancer, the molecular and cellular mechanism underlying Bacopa's nootropic action are not understood. In this study, we used deep sequencing (RNA-Seq) to identify the transcriptome changes upon Bacopa treatment on SH-SY5Y human neuroblastoma cells. We identified several genes whose expression levels were regulated by Bacopa. Biostatistical analysis of the RNA-Seq data identified biological pathways and molecular functions that were regulated by Bacopa, including regulation of mRNA translation and transmembrane transport, responses to oxidative stress and protein misfolding. Pathway analysis using the Ingenuity platform suggested that Bacopa may protect against brain damage and improve brain development. These newly identified molecular and cellular determinants may contribute to the nootropic action of Bacopa and open up a new direction of investigation into its mechanism of action.

Full Text

Duke Authors

Cited Authors

  • Leung, H-W; Foo, G; Banumurthy, G; Chai, X; Ghosh, S; Mitra-Ganguli, T; VanDongen, AMJ

Published Date

  • 2017

Published In

Volume / Issue

  • 12 / 8

Start / End Page

  • e0182984 -

PubMed ID

  • 28832626

Pubmed Central ID

  • PMC5568221

Electronic International Standard Serial Number (EISSN)

  • 1932-6203

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0182984


  • eng

Conference Location

  • United States