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Chemotaxis-driven assembly of endothelial barrier in a tumor-on-a-chip platform.

Publication ,  Journal Article
Aung, A; Theprungsirikul, J; Lim, HL; Varghese, S
Published in: Lab Chip
May 21, 2016

The integration of three-dimensional micropatterning with microfluidics provides a unique opportunity to create perfusable tissue constructs in vitro. Herein, we have used this approach to create a tumor-on-a-chip with an endothelial barrier. Specifically, we photopatterned a mixture of endothelial cells and cancer spheroids within a gelatin methacrylate (GelMA) hydrogel inside a microfluidic device. The differential motility of endothelial and cancer cells in response to a controlled morphogen gradient across the cell-laden network drove the migration of endothelial cells to the periphery while maintaining the cancer cells within the interior of the hydrogel. The resultant endothelial cell layer forming cell-cell contact via VE-cadherin junctions was found to encompass the entire GelMA hydrogel structure. Furthermore, we have also examined the potential of such a tumor-on-a-chip system as a drug screening platform using doxorubicin, a model cancer drug.

Duke Scholars

Published In

Lab Chip

DOI

EISSN

1473-0189

Publication Date

May 21, 2016

Volume

16

Issue

10

Start / End Page

1886 / 1898

Location

England

Related Subject Headings

  • Tumor Microenvironment
  • Spheroids, Cellular
  • Microfluidics
  • Methacrylates
  • MCF-7 Cells
  • Lab-On-A-Chip Devices
  • Humans
  • Human Umbilical Vein Endothelial Cells
  • Gelatin
  • Endothelial Cells
 

Citation

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MLA
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Aung, A., Theprungsirikul, J., Lim, H. L., & Varghese, S. (2016). Chemotaxis-driven assembly of endothelial barrier in a tumor-on-a-chip platform. Lab Chip, 16(10), 1886–1898. https://doi.org/10.1039/c6lc00184j
Aung, Aereas, Jomkuan Theprungsirikul, Han Liang Lim, and Shyni Varghese. “Chemotaxis-driven assembly of endothelial barrier in a tumor-on-a-chip platform.Lab Chip 16, no. 10 (May 21, 2016): 1886–98. https://doi.org/10.1039/c6lc00184j.
Aung A, Theprungsirikul J, Lim HL, Varghese S. Chemotaxis-driven assembly of endothelial barrier in a tumor-on-a-chip platform. Lab Chip. 2016 May 21;16(10):1886–98.
Aung, Aereas, et al. “Chemotaxis-driven assembly of endothelial barrier in a tumor-on-a-chip platform.Lab Chip, vol. 16, no. 10, May 2016, pp. 1886–98. Pubmed, doi:10.1039/c6lc00184j.
Aung A, Theprungsirikul J, Lim HL, Varghese S. Chemotaxis-driven assembly of endothelial barrier in a tumor-on-a-chip platform. Lab Chip. 2016 May 21;16(10):1886–1898.
Journal cover image

Published In

Lab Chip

DOI

EISSN

1473-0189

Publication Date

May 21, 2016

Volume

16

Issue

10

Start / End Page

1886 / 1898

Location

England

Related Subject Headings

  • Tumor Microenvironment
  • Spheroids, Cellular
  • Microfluidics
  • Methacrylates
  • MCF-7 Cells
  • Lab-On-A-Chip Devices
  • Humans
  • Human Umbilical Vein Endothelial Cells
  • Gelatin
  • Endothelial Cells