Chemotaxis-driven assembly of endothelial barrier in a tumor-on-a-chip platform.

Journal Article (Journal Article)

The integration of three-dimensional micropatterning with microfluidics provides a unique opportunity to create perfusable tissue constructs in vitro. Herein, we have used this approach to create a tumor-on-a-chip with an endothelial barrier. Specifically, we photopatterned a mixture of endothelial cells and cancer spheroids within a gelatin methacrylate (GelMA) hydrogel inside a microfluidic device. The differential motility of endothelial and cancer cells in response to a controlled morphogen gradient across the cell-laden network drove the migration of endothelial cells to the periphery while maintaining the cancer cells within the interior of the hydrogel. The resultant endothelial cell layer forming cell-cell contact via VE-cadherin junctions was found to encompass the entire GelMA hydrogel structure. Furthermore, we have also examined the potential of such a tumor-on-a-chip system as a drug screening platform using doxorubicin, a model cancer drug.

Full Text

Duke Authors

Cited Authors

  • Aung, A; Theprungsirikul, J; Lim, HL; Varghese, S

Published Date

  • May 21, 2016

Published In

Volume / Issue

  • 16 / 10

Start / End Page

  • 1886 - 1898

PubMed ID

  • 27097908

Pubmed Central ID

  • PMC4862883

Electronic International Standard Serial Number (EISSN)

  • 1473-0189

Digital Object Identifier (DOI)

  • 10.1039/c6lc00184j


  • eng

Conference Location

  • England