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Chondrogenic differentiation of human embryonic germ cell derived cells in hydrogels.

Publication ,  Journal Article
Varghese, S; Theprungsirikul, P; Ferran, A; Hwang, N; Canver, A; Elisseeff, J
Published in: Conf Proc IEEE Eng Med Biol Soc
2006

Human embryonic germ (hEG) cells have the potential to self-renew over long periods of time and differentiate into various lineages. Cells derived from embryoid bodies of hEG cells express a broad spectrum of gene markers and have been induced towards cells of ecto-dermal and recently endo-dermal and mesenchymal lineages. LVEC cells express a number of surface marker proteins characteristic of mesenchymal stem cells (MSCs), indicating the potential of these cells to differentiate into mesenchymal tissues. Here we demonstrate the homogenous differentiation of LVEC cells into hyaline cartilage. Three dimensional tissue formation is achieved by encapsulating cells in synthetic hydrogels followed by incubation in chondrocyte-conditioned culture medium. Homogenous hyaline cartilage was produced, even after 63 population doublings (13 passages). The high proliferative capacity of these cells without teratoma formation, homogenous differentiation, and three-dimensional cartilage tissue formation suggests the significant potential of LVEC cells for cartilage tissue engineering applications.

Duke Scholars

Published In

Conf Proc IEEE Eng Med Biol Soc

DOI

ISSN

1557-170X

Publication Date

2006

Volume

2006

Start / End Page

2643 / 2646

Location

United States

Related Subject Headings

  • Tissue Engineering
  • Hydrogels
  • Humans
  • Germ Cells
  • Embryonic Stem Cells
  • Chondrogenesis
  • Chondrocytes
  • Cells, Cultured
  • Cell Survival
  • Cell Proliferation
 

Citation

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ICMJE
MLA
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Varghese, S., Theprungsirikul, P., Ferran, A., Hwang, N., Canver, A., & Elisseeff, J. (2006). Chondrogenic differentiation of human embryonic germ cell derived cells in hydrogels. Conf Proc IEEE Eng Med Biol Soc, 2006, 2643–2646. https://doi.org/10.1109/IEMBS.2006.259710
Varghese, Shyni, Paranduangji Theprungsirikul, Angela Ferran, Nathaniel Hwang, Adam Canver, and Jennifer Elisseeff. “Chondrogenic differentiation of human embryonic germ cell derived cells in hydrogels.Conf Proc IEEE Eng Med Biol Soc 2006 (2006): 2643–46. https://doi.org/10.1109/IEMBS.2006.259710.
Varghese S, Theprungsirikul P, Ferran A, Hwang N, Canver A, Elisseeff J. Chondrogenic differentiation of human embryonic germ cell derived cells in hydrogels. Conf Proc IEEE Eng Med Biol Soc. 2006;2006:2643–6.
Varghese, Shyni, et al. “Chondrogenic differentiation of human embryonic germ cell derived cells in hydrogels.Conf Proc IEEE Eng Med Biol Soc, vol. 2006, 2006, pp. 2643–46. Pubmed, doi:10.1109/IEMBS.2006.259710.
Varghese S, Theprungsirikul P, Ferran A, Hwang N, Canver A, Elisseeff J. Chondrogenic differentiation of human embryonic germ cell derived cells in hydrogels. Conf Proc IEEE Eng Med Biol Soc. 2006;2006:2643–2646.

Published In

Conf Proc IEEE Eng Med Biol Soc

DOI

ISSN

1557-170X

Publication Date

2006

Volume

2006

Start / End Page

2643 / 2646

Location

United States

Related Subject Headings

  • Tissue Engineering
  • Hydrogels
  • Humans
  • Germ Cells
  • Embryonic Stem Cells
  • Chondrogenesis
  • Chondrocytes
  • Cells, Cultured
  • Cell Survival
  • Cell Proliferation