Chondrogenic differentiation of human embryonic germ cell derived cells in hydrogels.

Published

Journal Article

Human embryonic germ (hEG) cells have the potential to self-renew over long periods of time and differentiate into various lineages. Cells derived from embryoid bodies of hEG cells express a broad spectrum of gene markers and have been induced towards cells of ecto-dermal and recently endo-dermal and mesenchymal lineages. LVEC cells express a number of surface marker proteins characteristic of mesenchymal stem cells (MSCs), indicating the potential of these cells to differentiate into mesenchymal tissues. Here we demonstrate the homogenous differentiation of LVEC cells into hyaline cartilage. Three dimensional tissue formation is achieved by encapsulating cells in synthetic hydrogels followed by incubation in chondrocyte-conditioned culture medium. Homogenous hyaline cartilage was produced, even after 63 population doublings (13 passages). The high proliferative capacity of these cells without teratoma formation, homogenous differentiation, and three-dimensional cartilage tissue formation suggests the significant potential of LVEC cells for cartilage tissue engineering applications.

Full Text

Duke Authors

Cited Authors

  • Varghese, S; Theprungsirikul, P; Ferran, A; Hwang, N; Canver, A; Elisseeff, J

Published Date

  • January 2006

Published In

Volume / Issue

  • 1 /

Start / End Page

  • 2643 - 2646

PubMed ID

  • 17946525

Pubmed Central ID

  • 17946525

International Standard Serial Number (ISSN)

  • 1557-170X

Digital Object Identifier (DOI)

  • 10.1109/iembs.2006.259710

Language

  • eng