Long-term human pluripotent stem cell self-renewal on synthetic polymer surfaces.
Published
Journal Article
Realization of the full potential of human pluripotent stem cells (hPSCs) in regenerative medicine requires the development of well-defined culture conditions for their long-term growth and directed differentiation. Current practices for maintaining hPSCs generally utilize empirically determined combinations of feeder cells and other animal-based products, which are expensive, difficult to isolate, subject to batch-to-batch variations, and unsuitable for cell-based therapies. Using a high-throughput screening approach, we identified several polymers that can support self-renewal of hPSCs. While most of these polymers provide support for only a short period of time, we identified a synthetic polymer poly(methyl vinyl ether-alt-maleic anhydride) (PMVE-alt-MA) that supported the long-term attachment, proliferation and self-renewal of HUES1, HUES9, and iPSCs. The hPSCs cultured on PMVE-alt-MA maintained their characteristic morphology, expressed high levels of markers of pluripotency, and retained a normal karyotype. Such cost-effective, polymer-based matrices that support long-term self-renewal and proliferation of hPSCs will not only help to accelerate the translational perspectives of hPSCs, but also provide a platform to elucidate the underlying molecular mechanisms that regulate stem cell proliferation and differentiation.
Full Text
Duke Authors
Cited Authors
- Brafman, DA; Chang, CW; Fernandez, A; Willert, K; Varghese, S; Chien, S
Published Date
- December 2010
Published In
Volume / Issue
- 31 / 34
Start / End Page
- 9135 - 9144
PubMed ID
- 20817292
Pubmed Central ID
- 20817292
Electronic International Standard Serial Number (EISSN)
- 1878-5905
Digital Object Identifier (DOI)
- 10.1016/j.biomaterials.2010.08.007
Language
- eng
Conference Location
- Netherlands