Long Noncoding RNA Ceruloplasmin Promotes Cancer Growth by Altering Glycolysis.

Published

Journal Article

Long noncoding RNAs (lncRNAs) significantly influence the development and regulation of genome expression in cells. Here, we demonstrate the role of lncRNA ceruloplasmin (NRCP) in cancer metabolism and elucidate functional effects leading to increased tumor progression. NRCP was highly upregulated in ovarian tumors, and knockdown of NRCP resulted in significantly increased apoptosis, decreased cell proliferation, and decreased glycolysis compared with control cancer cells. In an orthotopic mouse model of ovarian cancer, siNRCP delivered via a liposomal carrier significantly reduced tumor growth compared with control treatment. We identified NRCP as an intermediate binding partner between STAT1 and RNA polymerase II, leading to increased expression of downstream target genes such as glucose-6-phosphate isomerase. Collectively, we report a previously unrecognized role of the lncRNA NRCP in modulating cancer metabolism. As demonstrated, DOPC nanoparticle-incorporated siRNA-mediated silencing of this lncRNA in vivo provides therapeutic avenue toward modulating lncRNAs in cancer.

Full Text

Duke Authors

Cited Authors

  • Rupaimoole, R; Lee, J; Haemmerle, M; Ling, H; Previs, RA; Pradeep, S; Wu, SY; Ivan, C; Ferracin, M; Dennison, JB; Millward, NMZ; Nagaraja, AS; Gharpure, KM; McGuire, M; Sam, N; Armaiz-Pena, GN; Sadaoui, NC; Rodriguez-Aguayo, C; Calin, GA; Drapkin, RI; Kovacs, J; Mills, GB; Zhang, W; Lopez-Berestein, G; Bhattacharya, PK; Sood, AK

Published Date

  • December 22, 2015

Published In

Volume / Issue

  • 13 / 11

Start / End Page

  • 2395 - 2402

PubMed ID

  • 26686630

Pubmed Central ID

  • 26686630

Electronic International Standard Serial Number (EISSN)

  • 2211-1247

Digital Object Identifier (DOI)

  • 10.1016/j.celrep.2015.11.047

Language

  • eng

Conference Location

  • United States