Gαi is required for carvedilol-induced β1 adrenergic receptor β-arrestin biased signaling.

Published online

Journal Article

The β1 adrenergic receptor (β1AR) is recognized as a classical Gαs-coupled receptor. Agonist binding not only initiates G protein-mediated signaling but also signaling through the multifunctional adapter protein β-arrestin. Some βAR ligands, such as carvedilol, stimulate βAR signaling preferentially through β-arrestin, a concept known as β-arrestin-biased agonism. Here, we identify a signaling mechanism, unlike that previously known for any Gαs-coupled receptor, whereby carvedilol induces the transition of the β1AR from a classical Gαs-coupled receptor to a Gαi-coupled receptor stabilizing a distinct receptor conformation to initiate β-arrestin-mediated signaling. Recruitment of Gαi is not induced by any other βAR ligand screened, nor is it required for β-arrestin-bias activated by the β2AR subtype of the βAR family. Our findings demonstrate a previously unrecognized role for Gαi in β1AR signaling and suggest that the concept of β-arrestin-bias may need to be refined to incorporate the selective bias of receptors towards distinct G protein subtypes.

Full Text

Duke Authors

Cited Authors

  • Wang, J; Hanada, K; Staus, DP; Makara, MA; Dahal, GR; Chen, Q; Ahles, A; Engelhardt, S; Rockman, HA

Published Date

  • November 22, 2017

Published In

Volume / Issue

  • 8 / 1

Start / End Page

  • 1706 -

PubMed ID

  • 29167435

Pubmed Central ID

  • 29167435

Electronic International Standard Serial Number (EISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/s41467-017-01855-z

Language

  • eng

Conference Location

  • England