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Intramolecular immunological signal hypothesis revived--structural background of signalling revealed by using Congo Red as a specific tool.

Publication ,  Journal Article
Jagusiak, A; Konieczny, L; Krol, M; Marszalek, P; Piekarska, B; Piwowar, P; Roterman, I; Rybarska, J; Stopa, B; Zemanek, G
Published in: Mini reviews in medicinal chemistry
January 2015

Micellar structures formed by self-assembling Congo red molecules bind to proteins penetrating into function-related unstable packing areas. Here, we have used Congo red--a supramolecular protein ligand--to investigate how the intramolecular structural changes that take place in antibodies following antigen binding lead to complement activation. According to our findings, Congo red binding significantly enhances the formation of antigen-antibody complexes. As a result, even low-affinity transiently binding antibodies can participate in immune complexes in the presence of Congo red, although immune complexes formed by these antibodies fail to trigger the complement cascade. This indicates that binding of antibodies to the antigen may not, by itself, fulfill the necessary conditions to generate the signal which triggers effector activity. These findings, together with the results of molecular dynamics simulation studies, enable us to conclude that, apart from the necessary assembling of antibodies, intramolecular structural changes generated by strains which associate high- affinity bivalent antibody fitting to antigen determinants are also required to cross the complement activation threshold.

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Published In

Mini reviews in medicinal chemistry

DOI

EISSN

1875-5607

ISSN

1389-5575

Publication Date

January 2015

Volume

14

Issue

13

Start / End Page

1104 / 1113

Related Subject Headings

  • Signal Transduction
  • Medicinal & Biomolecular Chemistry
  • Humans
  • Congo Red
  • Complement Activation
  • Antigen-Antibody Complex
  • Antibodies
  • 3404 Medicinal and biomolecular chemistry
  • 3214 Pharmacology and pharmaceutical sciences
  • 1115 Pharmacology and Pharmaceutical Sciences
 

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Jagusiak, A., Konieczny, L., Krol, M., Marszalek, P., Piekarska, B., Piwowar, P., … Zemanek, G. (2015). Intramolecular immunological signal hypothesis revived--structural background of signalling revealed by using Congo Red as a specific tool. Mini Reviews in Medicinal Chemistry, 14(13), 1104–1113. https://doi.org/10.2174/1389557514666141127150803
Jagusiak, A., L. Konieczny, M. Krol, P. Marszalek, B. Piekarska, P. Piwowar, I. Roterman, J. Rybarska, B. Stopa, and G. Zemanek. “Intramolecular immunological signal hypothesis revived--structural background of signalling revealed by using Congo Red as a specific tool.Mini Reviews in Medicinal Chemistry 14, no. 13 (January 2015): 1104–13. https://doi.org/10.2174/1389557514666141127150803.
Jagusiak A, Konieczny L, Krol M, Marszalek P, Piekarska B, Piwowar P, et al. Intramolecular immunological signal hypothesis revived--structural background of signalling revealed by using Congo Red as a specific tool. Mini reviews in medicinal chemistry. 2015 Jan;14(13):1104–13.
Jagusiak, A., et al. “Intramolecular immunological signal hypothesis revived--structural background of signalling revealed by using Congo Red as a specific tool.Mini Reviews in Medicinal Chemistry, vol. 14, no. 13, Jan. 2015, pp. 1104–13. Epmc, doi:10.2174/1389557514666141127150803.
Jagusiak A, Konieczny L, Krol M, Marszalek P, Piekarska B, Piwowar P, Roterman I, Rybarska J, Stopa B, Zemanek G. Intramolecular immunological signal hypothesis revived--structural background of signalling revealed by using Congo Red as a specific tool. Mini reviews in medicinal chemistry. 2015 Jan;14(13):1104–1113.

Published In

Mini reviews in medicinal chemistry

DOI

EISSN

1875-5607

ISSN

1389-5575

Publication Date

January 2015

Volume

14

Issue

13

Start / End Page

1104 / 1113

Related Subject Headings

  • Signal Transduction
  • Medicinal & Biomolecular Chemistry
  • Humans
  • Congo Red
  • Complement Activation
  • Antigen-Antibody Complex
  • Antibodies
  • 3404 Medicinal and biomolecular chemistry
  • 3214 Pharmacology and pharmaceutical sciences
  • 1115 Pharmacology and Pharmaceutical Sciences