Simple high-performance liquid chromatographic method to monitor vigabatrin, and preliminary review of concentrations determined in epileptic patients.

Published

Journal Article

A simple and rapid high-performance liquid chromatographic method has been developed for the determination of vigabatrin concentrations in plasma or serum. The assay uses only 100 microL of specimen and has been found to be linear over a concentration range of 1 to 50 mg/L. The limit of detection has been determined as 1 mg/L, and the between-batch coefficient of variation for the two internal quality controls routinely analysed (n = 33) has been found to be less than 5%. There was no evidence of interferences in the assay from other commonly prescribed anti-epileptic drugs. This method has been applied to routine clinical specimens to determine the concentration of vigabatrin in 47 patient specimens over a 12-month period. It was found that only 63% of the male group and 53% of the female group were within the proposed target concentration of 5 to 35 mg/L. In addition, it was found that 26% of the male group and 36% of the female group were found to have concentrations below 5 mg/L, which may indicate lack of compliance and/or lack of therapeutic efficacy of treatment.

Full Text

Duke Authors

Cited Authors

  • George, S; Gill, L; Braithwaite, RA

Published Date

  • May 2000

Published In

Volume / Issue

  • 37 ( Pt 3) /

Start / End Page

  • 338 - 342

PubMed ID

  • 10817248

Pubmed Central ID

  • 10817248

International Standard Serial Number (ISSN)

  • 0004-5632

Digital Object Identifier (DOI)

  • 10.1258/0004563001899249

Language

  • eng

Conference Location

  • England