Transcription-factor-dependent enhancer transcription defines a gene regulatory network for cardiac rhythm.
Journal Article (Journal Article)
The noncoding genome is pervasively transcribed. Noncoding RNAs (ncRNAs) generated from enhancers have been proposed as a general facet of enhancer function and some have been shown to be required for enhancer activity. Here we examine the transcription-factor-(TF)-dependence of ncRNA expression to define enhancers and enhancer-associated ncRNAs that are involved in a TF-dependent regulatory network. TBX5, a cardiac TF, regulates a network of cardiac channel genes to maintain cardiac rhythm. We deep sequenced wildtype and Tbx5-mutant mouse atria, identifying ~2600 novel Tbx5-dependent ncRNAs. Tbx5-dependent ncRNAs were enriched for tissue-specific marks of active enhancers genome-wide. Tbx5-dependent ncRNAs emanated from regions that are enriched for TBX5-binding and that demonstrated Tbx5-dependent enhancer activity. Tbx5-dependent ncRNA transcription provided a quantitative metric of Tbx5-dependent enhancer activity, correlating with target gene expression. We identified RACER, a novel Tbx5-dependent long noncoding RNA (lncRNA) required for the expression of the calcium-handling gene Ryr2. We illustrate that TF-dependent enhancer transcription can illuminate components of TF-dependent gene regulatory networks.
Full Text
Duke Authors
Cited Authors
- Yang, XH; Nadadur, RD; Hilvering, CR; Bianchi, V; Werner, M; Mazurek, SR; Gadek, M; Shen, KM; Goldman, JA; Tyan, L; Bekeny, J; Hall, JM; Lee, N; Perez-Cervantes, C; Burnicka-Turek, O; Poss, KD; Weber, CR; de Laat, W; Ruthenburg, AJ; Moskowitz, IP
Published Date
- December 27, 2017
Published In
Volume / Issue
- 6 /
PubMed ID
- 29280435
Pubmed Central ID
- PMC5745077
Electronic International Standard Serial Number (EISSN)
- 2050-084X
Digital Object Identifier (DOI)
- 10.7554/eLife.31683
Language
- eng
Conference Location
- England