CRISPR-based methods for high-throughput annotation of regulatory DNA.

Journal Article (Review;Journal Article)

Developments in CRISPR/Cas9-based technologies provide a new paradigm in functional screening of the genome. Conventional screening methods have focused on high-throughput perturbations of the protein-coding genome with technologies such as RNAi. However, equivalent methods for perturbing the non-coding genome have not existed until recently. CRISPR-based screening of genomic DNA has enabled the study of both genes and non-coding gene regulatory elements. Here we review recent progress in assigning function to the non-coding genome using CRISPR-based genomic and epigenomic screens, and discuss the prospects of these technologies to transforming our understanding of genome structure and regulation.

Full Text

Duke Authors

Cited Authors

  • Klann, TS; Black, JB; Gersbach, CA

Published Date

  • August 2018

Published In

Volume / Issue

  • 52 /

Start / End Page

  • 32 - 41

PubMed ID

  • 29500989

Pubmed Central ID

  • PMC6082715

Electronic International Standard Serial Number (EISSN)

  • 1879-0429

International Standard Serial Number (ISSN)

  • 0958-1669

Digital Object Identifier (DOI)

  • 10.1016/j.copbio.2018.02.004


  • eng