CX3CR1+ interstitial dendritic cells form a contiguous network throughout the entire kidney.
Journal Article
Dendritic cells (DCs) interface innate and adaptive immunity in nonlymphoid organs; however, the exact distribution and types of DC within the kidney are not known. We utilized CX3CR1GFP/+ mice to characterize the anatomy and phenotype of tissue-resident CX3CR1+ DCs within normal kidney. Laser-scanning confocal microscopy revealed an extensive, contiguous network of stellate-shaped CX3CR1+ DCs throughout the interstitial and mesangial spaces of the entire kidney. Intravital microscopy of the superficial cortex showed stationary interstitial CX3CR1+ DCs that continually probe the surrounding tissue environment through dendrite extensions. Flow cytometry of renal CX3CR1+ DCs showed significant coexpression of CD11c and F4/80, high major histocompatibility complex class II and FcR expression, and immature costimulatory but competent phagocytic ability indicative of tissue-resident, immature DCs ready to respond to environment cues. Thus, within the renal parenchyma, there exists little immunological privilege from the surveillance provided by renal CX3CR1+ DCs, a major constituent of the heterogeneous mononuclear phagocyte system populating normal kidney.
Full Text
Duke Authors
Cited Authors
- Soos, TJ; Sims, TN; Barisoni, L; Lin, K; Littman, DR; Dustin, ML; Nelson, PJ
Published Date
- August 2006
Published In
Volume / Issue
- 70 / 3
Start / End Page
- 591 - 596
PubMed ID
- 16760907
Pubmed Central ID
- 16760907
Electronic International Standard Serial Number (EISSN)
- 1523-1755
International Standard Serial Number (ISSN)
- 0085-2538
Digital Object Identifier (DOI)
- 10.1038/sj.ki.5001567
Language
- eng