CX3CR1+ interstitial dendritic cells form a contiguous network throughout the entire kidney.

Published

Journal Article

Dendritic cells (DCs) interface innate and adaptive immunity in nonlymphoid organs; however, the exact distribution and types of DC within the kidney are not known. We utilized CX3CR1GFP/+ mice to characterize the anatomy and phenotype of tissue-resident CX3CR1+ DCs within normal kidney. Laser-scanning confocal microscopy revealed an extensive, contiguous network of stellate-shaped CX3CR1+ DCs throughout the interstitial and mesangial spaces of the entire kidney. Intravital microscopy of the superficial cortex showed stationary interstitial CX3CR1+ DCs that continually probe the surrounding tissue environment through dendrite extensions. Flow cytometry of renal CX3CR1+ DCs showed significant coexpression of CD11c and F4/80, high major histocompatibility complex class II and FcR expression, and immature costimulatory but competent phagocytic ability indicative of tissue-resident, immature DCs ready to respond to environment cues. Thus, within the renal parenchyma, there exists little immunological privilege from the surveillance provided by renal CX3CR1+ DCs, a major constituent of the heterogeneous mononuclear phagocyte system populating normal kidney.

Full Text

Duke Authors

Cited Authors

  • Soos, TJ; Sims, TN; Barisoni, L; Lin, K; Littman, DR; Dustin, ML; Nelson, PJ

Published Date

  • August 2006

Published In

Volume / Issue

  • 70 / 3

Start / End Page

  • 591 - 596

PubMed ID

  • 16760907

Pubmed Central ID

  • 16760907

Electronic International Standard Serial Number (EISSN)

  • 1523-1755

International Standard Serial Number (ISSN)

  • 0085-2538

Digital Object Identifier (DOI)

  • 10.1038/sj.ki.5001567

Language

  • eng