An Adeno-Associated Virus-Based Toolkit for Preferential Targeting and Manipulating Quiescent Neural Stem Cells in the Adult Hippocampus.

Published

Journal Article

Quiescent neural stem cells (qNSCs) with radial morphology are the only proven source of new neurons in the adult mammalian brain. Our understanding of the roles of newly generated neurons depends on the ability to target and manipulate adult qNSCs. Although various strategies have been developed to target and manipulate adult hippocampal qNSCs, they often suffer from prolonged breeding, low recombination efficiency, and non-specific labeling. Therefore, developing a readily manufactured viral vector that allows flexible packaging and robust expression of various transgenes in qNSCs is a pressing need. Here, we report a recombinant adeno-associated virus serotype 4 (rAAV4)-based toolkit that preferentially targets hippocampal qNSCs and allows for lineage tracing, functional analyses, and activity manipulation of adult qNSCs. Importantly, targeting qNSCs in a non-Cre-dependent fashion opens the possibility for studying qNSCs in less genetically tractable animal species and may have translational impact in gene therapy by preferentially targeting qNSCs.

Full Text

Duke Authors

Cited Authors

  • Crowther, AJ; Lim, S-A; Asrican, B; Albright, BH; Wooten, J; Yeh, C-Y; Bao, H; Cerri, DH; Hu, J; Ian Shih, Y-Y; Asokan, A; Song, J

Published Date

  • March 2018

Published In

Volume / Issue

  • 10 / 3

Start / End Page

  • 1146 - 1159

PubMed ID

  • 29478897

Pubmed Central ID

  • 29478897

Electronic International Standard Serial Number (EISSN)

  • 2213-6711

International Standard Serial Number (ISSN)

  • 2213-6711

Digital Object Identifier (DOI)

  • 10.1016/j.stemcr.2018.01.018

Language

  • eng