Production of recombinant adeno-associated viral vectors and use for in vitro and in vivo administration.

Published

Journal Article

Adeno-associated virus is a nonpathogenic human virus that has been developed into a gene-delivery vector due to its high efficiency of infection for many different cell types and its ability to persist and lead to long-term gene expression. This unit describes efficient methods to generate high-titer, research-grade, adenovirus-free recombinant single-stranded and self-complementary adeno-associated virus in various serotypes, along with methods to quantify the viral vectors. Two detailed methods are provided for viral vector injection into rat brain and for histological detection of transgene expression, including lacZ and GFP.

Full Text

Duke Authors

Cited Authors

  • Choi, VW; Asokan, A; Haberman, RA; McCown, TJ; Samulski, RJ

Published Date

  • May 2006

Published In

Volume / Issue

  • Chapter 4 /

Start / End Page

  • Unit - 4.17

PubMed ID

  • 18428636

Pubmed Central ID

  • 18428636

Electronic International Standard Serial Number (EISSN)

  • 1934-8576

Digital Object Identifier (DOI)

  • 10.1002/0471142301.ns0417s35

Language

  • eng

Conference Location

  • United States