Production of recombinant adeno-associated viral vectors.
Publication
, Journal Article
Choi, VW; Asokan, A; Haberman, RA; Samulski, RJ
Published in: Curr Protoc Hum Genet
April 2007
Adeno-associated virus is a nonpathogenic human virus that has been developed into a gene-delivery vector due to its high efficiency of infection for many different cell types and its ability to persist and lead to long-term gene expression. This unit describes efficient methods to generate high-titer, research-grade, adenovirus-free recombinant single-stranded and self-complementary adeno-associated virus in various serotypes, along with methods to quantify the viral vectors.
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Published In
Curr Protoc Hum Genet
DOI
EISSN
1934-8258
Publication Date
April 2007
Volume
Chapter 12
Start / End Page
Unit / 12.9
Location
United States
Related Subject Headings
- Virus Cultivation
- Humans
- Genetics & Heredity
- Genetic Vectors
- Gene Transfer Techniques
- Dependovirus
- Animals
Citation
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Choi, V. W., Asokan, A., Haberman, R. A., & Samulski, R. J. (2007). Production of recombinant adeno-associated viral vectors. Curr Protoc Hum Genet, Chapter 12, Unit-12.9. https://doi.org/10.1002/0471142905.hg1209s53
Choi, Vivian W., Aravind Asokan, Rebecca A. Haberman, and Richard Jude Samulski. “Production of recombinant adeno-associated viral vectors.” Curr Protoc Hum Genet Chapter 12 (April 2007): Unit-12.9. https://doi.org/10.1002/0471142905.hg1209s53.
Choi VW, Asokan A, Haberman RA, Samulski RJ. Production of recombinant adeno-associated viral vectors. Curr Protoc Hum Genet. 2007 Apr;Chapter 12:Unit-12.9.
Choi, Vivian W., et al. “Production of recombinant adeno-associated viral vectors.” Curr Protoc Hum Genet, vol. Chapter 12, Apr. 2007, p. Unit-12.9. Pubmed, doi:10.1002/0471142905.hg1209s53.
Choi VW, Asokan A, Haberman RA, Samulski RJ. Production of recombinant adeno-associated viral vectors. Curr Protoc Hum Genet. 2007 Apr;Chapter 12:Unit-12.9.
Published In
Curr Protoc Hum Genet
DOI
EISSN
1934-8258
Publication Date
April 2007
Volume
Chapter 12
Start / End Page
Unit / 12.9
Location
United States
Related Subject Headings
- Virus Cultivation
- Humans
- Genetics & Heredity
- Genetic Vectors
- Gene Transfer Techniques
- Dependovirus
- Animals