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Lipopolysaccharide-induced expression of TRAIL promotes dendritic cell differentiation.

Publication ,  Journal Article
Cho, YS; Challa, S; Clancy, L; Chan, FK-M
Published in: Immunology
August 2010

Tumour necrosis factor-related apoptosis inducing ligand (TRAIL) is a death-inducing cytokine whose physiological function is not well understood. Here, we show that TRAIL has a role in programming human dendritic cell (DC) differentiation. TRAIL expression was strongly induced in DCs upon stimulation with lipopolysaccharide (LPS) or Polyinosine-polycytidylic acid (poly(I:C)) stimulation. Blockade of TRAIL with neutralizing antibody partially inhibited LPS-induced up-regulation of co-stimulatory molecules and the expression of inflammatory cytokines including interleukin-12 (IL-12) p70. In addition, neutralization of TRAIL in LPS-treated DCs inhibited the DC-driven differentiation of T cells into interferon-gamma (IFN-gamma) -producing effectors. The effects of TRAIL neutralization in poly(I:C)-treated DCs were similar, except that IL-12 production and the differentiation of effector T cells into IFN-gamma producers were not inhibited. Strikingly, TRAIL stimulation alone was sufficient to induce morphological changes resembling DC maturation, up-regulation of co-stimulatory molecules, and enhancement of DC-driven allogeneic T-cell proliferation. However, TRAIL alone did not induce inflammatory cytokine production. We further show that the effects of TRAIL on DC maturation were not the result of the induction of apoptosis, but may involve p38 activation. Hence, our data demonstrate that TRAIL co-operates with other cytokines to facilitate DC functional maturation in response to Toll-like receptor activation.

Duke Scholars

Published In

Immunology

DOI

EISSN

1365-2567

Publication Date

August 2010

Volume

130

Issue

4

Start / End Page

504 / 515

Location

England

Related Subject Headings

  • TNF-Related Apoptosis-Inducing Ligand
  • Signal Transduction
  • Lipopolysaccharides
  • Immunology
  • Humans
  • Dendritic Cells
  • Cytokines
  • Cells, Cultured
  • Cell Differentiation
  • Apoptosis
 

Citation

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ICMJE
MLA
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Cho, Y. S., Challa, S., Clancy, L., & Chan, F.-M. (2010). Lipopolysaccharide-induced expression of TRAIL promotes dendritic cell differentiation. Immunology, 130(4), 504–515. https://doi.org/10.1111/j.1365-2567.2010.03266.x
Cho, Young S., Sreerupa Challa, Lauren Clancy, and Francis K-M Chan. “Lipopolysaccharide-induced expression of TRAIL promotes dendritic cell differentiation.Immunology 130, no. 4 (August 2010): 504–15. https://doi.org/10.1111/j.1365-2567.2010.03266.x.
Cho YS, Challa S, Clancy L, Chan FK-M. Lipopolysaccharide-induced expression of TRAIL promotes dendritic cell differentiation. Immunology. 2010 Aug;130(4):504–15.
Cho, Young S., et al. “Lipopolysaccharide-induced expression of TRAIL promotes dendritic cell differentiation.Immunology, vol. 130, no. 4, Aug. 2010, pp. 504–15. Pubmed, doi:10.1111/j.1365-2567.2010.03266.x.
Cho YS, Challa S, Clancy L, Chan FK-M. Lipopolysaccharide-induced expression of TRAIL promotes dendritic cell differentiation. Immunology. 2010 Aug;130(4):504–515.
Journal cover image

Published In

Immunology

DOI

EISSN

1365-2567

Publication Date

August 2010

Volume

130

Issue

4

Start / End Page

504 / 515

Location

England

Related Subject Headings

  • TNF-Related Apoptosis-Inducing Ligand
  • Signal Transduction
  • Lipopolysaccharides
  • Immunology
  • Humans
  • Dendritic Cells
  • Cytokines
  • Cells, Cultured
  • Cell Differentiation
  • Apoptosis