A role for the orphan steroid receptor Nur77 in apoptosis accompanying antigen-induced negative selection.

Published

Journal Article

The transcription factor Nur77, an orphan member of the nuclear hormone receptor superfamily, is highly expressed during T cell receptor-signaled apoptosis, suggesting a possible role for Nur77 in negative selection. We examined this by generating two sets of transgenic mice. In one set of mice, a dominant-negative Nur77 mutant is constitutively expressed and the other in which wild-type Nur77 protein is constitutively expressed in developing thymocytes. We report that inhibition of endogenous Nur77 by the dominant-negative mutant perturbed T cell development and inhibited antigen-induced negative selection in F5T cell receptor transgenic mice. Constitutive expression of wild-type Nur77 protein induced apoptosis in developing thymocytes, resulting in a decreased number of thymocytes and mature T cells. Together, these data support a role for Nur77 in the downstream signaling events in antigen-induced negative selection.

Full Text

Duke Authors

Cited Authors

  • Calnan, BJ; Szychowski, S; Chan, FK; Cado, D; Winoto, A

Published Date

  • September 1995

Published In

Volume / Issue

  • 3 / 3

Start / End Page

  • 273 - 282

PubMed ID

  • 7552993

Pubmed Central ID

  • 7552993

Electronic International Standard Serial Number (EISSN)

  • 1097-4180

International Standard Serial Number (ISSN)

  • 1074-7613

Digital Object Identifier (DOI)

  • 10.1016/1074-7613(95)90113-2

Language

  • eng