Skip to main content
Journal cover image

Production of interleukin-6 in contracting human skeletal muscles can account for the exercise-induced increase in plasma interleukin-6.

Publication ,  Journal Article
Steensberg, A; van Hall, G; Osada, T; Sacchetti, M; Saltin, B; Klarlund Pedersen, B
Published in: J Physiol
November 15, 2000

1. Plasma interleukin (IL)-6 concentration is increased with exercise and it has been demonstrated that contracting muscles can produce IL-The question addressed in the present study was whether the IL-6 production by contracting skeletal muscle is of such a magnitude that it can account for the IL-6 accumulating in the blood. 2. This was studied in six healthy males, who performed one-legged dynamic knee extensor exercise for 5 h at 25 W, which represented 40% of peak power output (Wmax). Arterial-femoral venous (a-fv) differences over the exercising and the resting leg were obtained before and every hour during the exercise. Leg blood flow was measured in parallel by the ultrasound Doppler technique. IL-6 was measured by enzyme-linked immunosorbent assay (ELISA). 3. Arterial plasma concentrations for IL-6 increased 19-fold compared to rest. The a-fv difference for IL-6 over the exercising leg followed the same pattern as did the net IL-6 release. Over the resting leg, there was no significant a-fv difference or net IL-6 release. The work was produced by 2.5 kg of active muscle, which means that during the last 2 h of exercise, the median IL-6 production was 6.8 ng min-1 (kg active muscle)-1 (range, 3.96-9.69 ng min-1 kg-1). 4. The net IL-6 release from the muscle over the last 2 h of exercise was 17-fold higher than the elevation in arterial IL-6 concentration and at 5 h of exercise the net release during 1 min was half of the IL-6 content in the plasma. This indicates a very high turnover of IL-6 during muscular exercise. We suggest that IL-6 produced by skeletal contracting muscle contributes to the maintenance of glucose homeostasis during prolonged exercise.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Physiol

DOI

ISSN

0022-3751

Publication Date

November 15, 2000

Volume

529 Pt 1

Issue

Pt 1

Start / End Page

237 / 242

Location

England

Related Subject Headings

  • Time Factors
  • Regional Blood Flow
  • Physiology
  • Muscle, Skeletal
  • Muscle Contraction
  • Male
  • Leg
  • Interleukin-6
  • Humans
  • Exercise
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Steensberg, A., van Hall, G., Osada, T., Sacchetti, M., Saltin, B., & Klarlund Pedersen, B. (2000). Production of interleukin-6 in contracting human skeletal muscles can account for the exercise-induced increase in plasma interleukin-6. J Physiol, 529 Pt 1(Pt 1), 237–242. https://doi.org/10.1111/j.1469-7793.2000.00237.x
Steensberg, A., G. van Hall, T. Osada, M. Sacchetti, B. Saltin, and B. Klarlund Pedersen. “Production of interleukin-6 in contracting human skeletal muscles can account for the exercise-induced increase in plasma interleukin-6.J Physiol 529 Pt 1, no. Pt 1 (November 15, 2000): 237–42. https://doi.org/10.1111/j.1469-7793.2000.00237.x.
Steensberg A, van Hall G, Osada T, Sacchetti M, Saltin B, Klarlund Pedersen B. Production of interleukin-6 in contracting human skeletal muscles can account for the exercise-induced increase in plasma interleukin-6. J Physiol. 2000 Nov 15;529 Pt 1(Pt 1):237–42.
Steensberg, A., et al. “Production of interleukin-6 in contracting human skeletal muscles can account for the exercise-induced increase in plasma interleukin-6.J Physiol, vol. 529 Pt 1, no. Pt 1, Nov. 2000, pp. 237–42. Pubmed, doi:10.1111/j.1469-7793.2000.00237.x.
Steensberg A, van Hall G, Osada T, Sacchetti M, Saltin B, Klarlund Pedersen B. Production of interleukin-6 in contracting human skeletal muscles can account for the exercise-induced increase in plasma interleukin-6. J Physiol. 2000 Nov 15;529 Pt 1(Pt 1):237–242.
Journal cover image

Published In

J Physiol

DOI

ISSN

0022-3751

Publication Date

November 15, 2000

Volume

529 Pt 1

Issue

Pt 1

Start / End Page

237 / 242

Location

England

Related Subject Headings

  • Time Factors
  • Regional Blood Flow
  • Physiology
  • Muscle, Skeletal
  • Muscle Contraction
  • Male
  • Leg
  • Interleukin-6
  • Humans
  • Exercise