ROBO4 variants predispose individuals to bicuspid aortic valve and thoracic aortic aneurysm.
Bicuspid aortic valve (BAV) is a common congenital heart defect (population incidence, 1-2%)1-3 that frequently presents with ascending aortic aneurysm (AscAA)4. BAV/AscAA shows autosomal dominant inheritance with incomplete penetrance and male predominance. Causative gene mutations (for example, NOTCH1, SMAD6) are known for ≤1% of nonsyndromic BAV cases with and without AscAA5-8, impeding mechanistic insight and development of therapeutic strategies. Here, we report the identification of variants in ROBO4 (which encodes a factor known to contribute to endothelial performance) that segregate with disease in two families. Targeted sequencing of ROBO4 showed enrichment for rare variants in BAV/AscAA probands compared with controls. Targeted silencing of ROBO4 or mutant ROBO4 expression in endothelial cell lines results in impaired barrier function and a synthetic repertoire suggestive of endothelial-to-mesenchymal transition. This is consistent with BAV/AscAA-associated findings in patients and in animal models deficient for ROBO4. These data identify a novel endothelial etiology for this common human disease phenotype.
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- Zebrafish
- Receptors, Cell Surface
- Phenotype
- Mutation
- Mice, Knockout
- Mice, Inbred C57BL
- Mice
- Male
- Humans
- Heart Valve Diseases
Citation
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Zebrafish
- Receptors, Cell Surface
- Phenotype
- Mutation
- Mice, Knockout
- Mice, Inbred C57BL
- Mice
- Male
- Humans
- Heart Valve Diseases