Fluorescent peptide displacement as a general assay for screening small molecule libraries against RNA.
Journal Article (Journal Article)
A prominent hurdle in developing small molecule probes against RNA is the relative scarcity of general screening methods. In this study, we demonstrate the application of a fluorescent peptide displacement assay to screen small molecule probes against four different RNA targets. The designed experimental protocol combined with statistical analysis provides a fast and convenient method to simultaneously evaluate small molecule libraries against different RNA targets and classify them based on affinity and selectivity patterns.
- Patwardhan, NN; Cai, Z; Newson, CN; Hargrove, AE
- February 2019
Volume / Issue
- 17 / 7
Start / End Page
- 1778 - 1786
Pubmed Central ID
Electronic International Standard Serial Number (EISSN)
International Standard Serial Number (ISSN)
Digital Object Identifier (DOI)