Fluorescent peptide displacement as a general assay for screening small molecule libraries against RNA.

Journal Article (Journal Article)

A prominent hurdle in developing small molecule probes against RNA is the relative scarcity of general screening methods. In this study, we demonstrate the application of a fluorescent peptide displacement assay to screen small molecule probes against four different RNA targets. The designed experimental protocol combined with statistical analysis provides a fast and convenient method to simultaneously evaluate small molecule libraries against different RNA targets and classify them based on affinity and selectivity patterns.

Full Text

Duke Authors

Cited Authors

  • Patwardhan, NN; Cai, Z; Newson, CN; Hargrove, AE

Published Date

  • February 2019

Published In

Volume / Issue

  • 17 / 7

Start / End Page

  • 1778 - 1786

PubMed ID

  • 30468226

Pubmed Central ID

  • PMC6503966

Electronic International Standard Serial Number (EISSN)

  • 1477-0539

International Standard Serial Number (ISSN)

  • 1477-0520

Digital Object Identifier (DOI)

  • 10.1039/c8ob02467g


  • eng