Fluorescent peptide displacement as a general assay for screening small molecule libraries against RNA.
Publication
, Journal Article
Patwardhan, NN; Cai, Z; Newson, CN; Hargrove, AE
Published in: Organic & biomolecular chemistry
February 2019
A prominent hurdle in developing small molecule probes against RNA is the relative scarcity of general screening methods. In this study, we demonstrate the application of a fluorescent peptide displacement assay to screen small molecule probes against four different RNA targets. The designed experimental protocol combined with statistical analysis provides a fast and convenient method to simultaneously evaluate small molecule libraries against different RNA targets and classify them based on affinity and selectivity patterns.
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Published In
Organic & biomolecular chemistry
DOI
EISSN
1477-0539
ISSN
1477-0520
Publication Date
February 2019
Volume
17
Issue
7
Start / End Page
1778 / 1786
Related Subject Headings
- Small Molecule Libraries
- RNA
- Peptides
- Organic Chemistry
- Gene Products, tat
- Fluorescent Dyes
- Drug Evaluation, Preclinical
- Base Sequence
- Amino Acid Sequence
- 3405 Organic chemistry
Citation
APA
Chicago
ICMJE
MLA
NLM
Patwardhan, N. N., Cai, Z., Newson, C. N., & Hargrove, A. E. (2019). Fluorescent peptide displacement as a general assay for screening small molecule libraries against RNA. Organic & Biomolecular Chemistry, 17(7), 1778–1786. https://doi.org/10.1039/c8ob02467g
Patwardhan, Neeraj N., Zhengguo Cai, Colby N. Newson, and Amanda E. Hargrove. “Fluorescent peptide displacement as a general assay for screening small molecule libraries against RNA.” Organic & Biomolecular Chemistry 17, no. 7 (February 2019): 1778–86. https://doi.org/10.1039/c8ob02467g.
Patwardhan NN, Cai Z, Newson CN, Hargrove AE. Fluorescent peptide displacement as a general assay for screening small molecule libraries against RNA. Organic & biomolecular chemistry. 2019 Feb;17(7):1778–86.
Patwardhan, Neeraj N., et al. “Fluorescent peptide displacement as a general assay for screening small molecule libraries against RNA.” Organic & Biomolecular Chemistry, vol. 17, no. 7, Feb. 2019, pp. 1778–86. Epmc, doi:10.1039/c8ob02467g.
Patwardhan NN, Cai Z, Newson CN, Hargrove AE. Fluorescent peptide displacement as a general assay for screening small molecule libraries against RNA. Organic & biomolecular chemistry. 2019 Feb;17(7):1778–1786.
Published In
Organic & biomolecular chemistry
DOI
EISSN
1477-0539
ISSN
1477-0520
Publication Date
February 2019
Volume
17
Issue
7
Start / End Page
1778 / 1786
Related Subject Headings
- Small Molecule Libraries
- RNA
- Peptides
- Organic Chemistry
- Gene Products, tat
- Fluorescent Dyes
- Drug Evaluation, Preclinical
- Base Sequence
- Amino Acid Sequence
- 3405 Organic chemistry