Longitudinal stability in cigarette smokers of urinary biomarkers of exposure to the toxicants acrylonitrile and acrolein.

Journal Article (Journal Article)

The urinary metabolites cyanoethyl mercapturic acid (CEMA) and 3-hydroxypropyl mercapturic acid (3-HPMA) have been widely used as biomarkers of exposure to acrylonitrile and acrolein, respectively, but there are no published data on their consistency over time in the urine of cigarette smokers. We provided, free of charge over a 20 week period, Spectrum NRC600/601 research cigarettes to cigarette smokers in the control arm of a randomized clinical trial of the reduced nicotine cigarette. Urine samples were collected at weeks 4, 8, 12, 16, and 20 and analyzed for CEMA and 3-HPMA, and total nicotine equivalents (TNE) using validated methods. Creatinine-corrected intra-class correlation coefficients for CEMA, 3-HPMA, and TNE were 0.67, 0.46, and 0.68, respectively, indicating good longitudinal consistency for CEMA, while that of 3-HPMA was fair. A strong correlation between CEMA and TNE values was observed. These data support the use of CEMA as a reliable biomarker of tobacco smoke exposure. This is the first report of the longitudinal stability of the biomarkers of acrylonitrile and acrolein exposure in smokers. The data indicate that CEMA, the biomarker of acrylonitrile exposure, is consistent over time in cigarette smokers, supporting its use. While 3-HPMA levels were less stable over time, this biomarker is nevertheless a useful monitor of human acrolein exposure because of its specificity to this toxicant.

Full Text

Duke Authors

Cited Authors

  • Chen, M; Carmella, SG; Sipe, C; Jensen, J; Luo, X; Le, CT; Murphy, SE; Benowitz, NL; McClernon, FJ; Vandrey, R; Allen, SS; Denlinger-Apte, R; Cinciripini, PM; Strasser, AA; al'Absi, M; Robinson, JD; Donny, EC; Hatsukami, D; Hecht, SS

Published Date

  • 2019

Published In

Volume / Issue

  • 14 / 1

Start / End Page

  • e0210104 -

PubMed ID

  • 30608961

Pubmed Central ID

  • PMC6319718

Electronic International Standard Serial Number (EISSN)

  • 1932-6203

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0210104


  • eng

Conference Location

  • United States