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Diphenyl Phosphate-Induced Toxicity During Embryonic Development.

Publication ,  Journal Article
Mitchell, CA; Reddam, A; Dasgupta, S; Zhang, S; Stapleton, HM; Volz, DC
Published in: Environmental science & technology
April 2019

Diphenyl phosphate (DPHP) is an aryl phosphate ester (APE) used as an industrial catalyst and chemical additive and is the primary metabolite of flame retardant APEs, including triphenyl phosphate (TPHP). Minimal DPHP-specific toxicity studies have been published despite ubiquitous exposure within human populations following metabolism of TPHP and other APEs. Therefore, the objective of this study was to determine the potential for DPHP-induced toxicity during embryonic development. Using zebrafish as a model, we found that DPHP significantly increased the distance between the sinus venosus and bulbus arteriosis (SV-BA) at 72 h postfertilization (hpf) following initiation of exposure before and after cardiac looping. Interestingly, pretreatment with d-mannitol mitigated DPHP-induced effects on SV-BA length despite the absence of DPHP effects on pericardial area, suggesting that DPHP-induced cardiac defects are independent of pericardial edema formation. Using mRNA-sequencing, we found that DPHP disrupts pathways related to mitochondrial function and heme biosynthesis; indeed, DPHP significantly decreased hemoglobin levels in situ at 72 hpf following exposure from 24 to 72 hpf. Overall, our findings suggest that, similar to TPHP, DPHP impacts cardiac development, albeit the potency of DPHP is significantly less than TPHP within developing zebrafish.

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Published In

Environmental science & technology

DOI

EISSN

1520-5851

ISSN

0013-936X

Publication Date

April 2019

Volume

53

Issue

7

Start / End Page

3908 / 3916

Related Subject Headings

  • Phosphates
  • Humans
  • Flame Retardants
  • Environmental Sciences
  • Embryonic Development
  • Biphenyl Compounds
  • Animals
 

Citation

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Mitchell, C. A., Reddam, A., Dasgupta, S., Zhang, S., Stapleton, H. M., & Volz, D. C. (2019). Diphenyl Phosphate-Induced Toxicity During Embryonic Development. Environmental Science & Technology, 53(7), 3908–3916. https://doi.org/10.1021/acs.est.8b07238
Mitchell, Constance A., Aalekhya Reddam, Subham Dasgupta, Sharon Zhang, Heather M. Stapleton, and David C. Volz. “Diphenyl Phosphate-Induced Toxicity During Embryonic Development.Environmental Science & Technology 53, no. 7 (April 2019): 3908–16. https://doi.org/10.1021/acs.est.8b07238.
Mitchell CA, Reddam A, Dasgupta S, Zhang S, Stapleton HM, Volz DC. Diphenyl Phosphate-Induced Toxicity During Embryonic Development. Environmental science & technology. 2019 Apr;53(7):3908–16.
Mitchell, Constance A., et al. “Diphenyl Phosphate-Induced Toxicity During Embryonic Development.Environmental Science & Technology, vol. 53, no. 7, Apr. 2019, pp. 3908–16. Epmc, doi:10.1021/acs.est.8b07238.
Mitchell CA, Reddam A, Dasgupta S, Zhang S, Stapleton HM, Volz DC. Diphenyl Phosphate-Induced Toxicity During Embryonic Development. Environmental science & technology. 2019 Apr;53(7):3908–3916.
Journal cover image

Published In

Environmental science & technology

DOI

EISSN

1520-5851

ISSN

0013-936X

Publication Date

April 2019

Volume

53

Issue

7

Start / End Page

3908 / 3916

Related Subject Headings

  • Phosphates
  • Humans
  • Flame Retardants
  • Environmental Sciences
  • Embryonic Development
  • Biphenyl Compounds
  • Animals