Detecting and characterizing circular RNAs.

Journal Article (Journal Article)

Circular RNA transcripts were first identified in the early 1990s but knowledge of these species has remained limited, as their study through traditional methods of RNA analysis has been difficult. Now, novel bioinformatic approaches coupled with biochemical enrichment strategies and deep sequencing have allowed comprehensive studies of circular RNA species. Recent studies have revealed thousands of endogenous circular RNAs in mammalian cells, some of which are highly abundant and evolutionarily conserved. Evidence is emerging that some circRNAs might regulate microRNA (miRNA) function, and roles in transcriptional control have also been suggested. Therefore, study of this class of noncoding RNAs has potential implications for therapeutic and research applications. We believe the key future challenge for the field will be to understand the regulation and function of these unusual molecules.

Full Text

Duke Authors

Cited Authors

  • Jeck, WR; Sharpless, NE

Published Date

  • May 2014

Published In

Volume / Issue

  • 32 / 5

Start / End Page

  • 453 - 461

PubMed ID

  • 24811520

Pubmed Central ID

  • PMC4121655

Electronic International Standard Serial Number (EISSN)

  • 1546-1696

Digital Object Identifier (DOI)

  • 10.1038/nbt.2890


  • eng

Conference Location

  • United States