Ocular anterior chamber blood cell population differentiation using spectroscopic optical coherence tomography.

Journal Article (Journal Article)

There is potential clinical significance in identifying cellular responses in the anterior chamber (AC) of the eye, which can indicate hyphema (an accumulation of red blood cells [RBCs]) or aberrant intraocular inflammation (an accumulation of white blood cells [WBCs]). In this work, we developed a spectroscopic OCT analysis method to differentiate between populations of RBCs and subtypes of WBCs, including granulocytes, lymphocytes and monocytes, both in vitro and in ACs of porcine eyes. We developed an algorithm to track single cells within OCT data sets, and extracted the backscatter reflectance spectrum of each single cell from the detected interferograms using the short-time Fourier transform (STFT). A look-up table of Mie back-scattering spectra was generated and used to correlate the backscatter spectral features of single cells to their characteristic sizes. The extracted size distributions based on the best Mie spectra fit were significantly different between each cell type. We also studied theoretical backscattering models of single RBCs to further validate our experimental results. The described work is a promising step towards clinically differentiating and quantifying AC blood cell types.

Full Text

Duke Authors

Cited Authors

  • Qian, R; Huang, W-F; McNabb, RP; Zhou, KC; Liu, QH; Kuo, AN; Izatt, JA

Published Date

  • July 1, 2019

Published In

Volume / Issue

  • 10 / 7

Start / End Page

  • 3281 - 3300

PubMed ID

  • 31467779

Pubmed Central ID

  • PMC6706034

International Standard Serial Number (ISSN)

  • 2156-7085

Digital Object Identifier (DOI)

  • 10.1364/BOE.10.003281


  • eng

Conference Location

  • United States