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Simultaneous photoacoustic imaging of intravascular and tissue oxygenation.

Publication ,  Journal Article
Chen, M; Knox, HJ; Tang, Y; Liu, W; Nie, L; Chan, J; Yao, J
Published in: Optics letters
August 2019

Hypoxia, a low tissue oxygenation condition caused by insufficient oxygen supply, leads to potentially irreversible tissue damage, such as brain infarction during stroke. Intravascular oxygenation has long been used by photoacoustic imaging, among other imaging modalities, to study hypoxia. However, intravascular oxygenation describes only the oxygen supply via microcirculation, which does not directly reflect the amount of free oxygen available for metabolism in the interstitial fluid. Therefore, to fully understand hypoxia, it is highly desirable to monitor blood oxygenation as well as tissue oxygenation during the same biological process. In this work, by combining high-resolution photoacoustic microscopy (PAM) and a novel bioreducible N-oxide-based hypoxia-sensitive probe HyP-650, we have demonstrated simultaneous imaging of intravascular oxygenation and tissue hypoxia. We have established detailed chemical, optical, and photoacoustic properties of HyP-650 for hypoxic activation in vitro and in living cells. We have also performed PAM on hindlimb ischemia models and tumor-bearing mice to study the correlation between intravascular oxygenation and tissue oxygenation at various hypoxic levels. We expect that Hyp-650 enhanced photoacoustic imaging will find a variety of applications in brain and cancer research.

Duke Scholars

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Published In

Optics letters

DOI

EISSN

1539-4794

ISSN

0146-9592

Publication Date

August 2019

Volume

44

Issue

15

Start / End Page

3773 / 3776

Related Subject Headings

  • Tumor Hypoxia
  • Photoacoustic Techniques
  • Oxygen
  • Optics
  • Microscopy
  • Mice
  • Ischemia
  • Hindlimb
  • Blood Vessels
  • Animals
 

Citation

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ICMJE
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Chen, M., Knox, H. J., Tang, Y., Liu, W., Nie, L., Chan, J., & Yao, J. (2019). Simultaneous photoacoustic imaging of intravascular and tissue oxygenation. Optics Letters, 44(15), 3773–3776. https://doi.org/10.1364/ol.44.003773
Chen, Maomao, Hailey J. Knox, Yuqi Tang, Wei Liu, Liming Nie, Jefferson Chan, and Junjie Yao. “Simultaneous photoacoustic imaging of intravascular and tissue oxygenation.Optics Letters 44, no. 15 (August 2019): 3773–76. https://doi.org/10.1364/ol.44.003773.
Chen M, Knox HJ, Tang Y, Liu W, Nie L, Chan J, et al. Simultaneous photoacoustic imaging of intravascular and tissue oxygenation. Optics letters. 2019 Aug;44(15):3773–6.
Chen, Maomao, et al. “Simultaneous photoacoustic imaging of intravascular and tissue oxygenation.Optics Letters, vol. 44, no. 15, Aug. 2019, pp. 3773–76. Epmc, doi:10.1364/ol.44.003773.
Chen M, Knox HJ, Tang Y, Liu W, Nie L, Chan J, Yao J. Simultaneous photoacoustic imaging of intravascular and tissue oxygenation. Optics letters. 2019 Aug;44(15):3773–3776.
Journal cover image

Published In

Optics letters

DOI

EISSN

1539-4794

ISSN

0146-9592

Publication Date

August 2019

Volume

44

Issue

15

Start / End Page

3773 / 3776

Related Subject Headings

  • Tumor Hypoxia
  • Photoacoustic Techniques
  • Oxygen
  • Optics
  • Microscopy
  • Mice
  • Ischemia
  • Hindlimb
  • Blood Vessels
  • Animals