Forward and Reverse Genetic Analysis of Chlamydia.

Book Section

Chlamydia is a major etiological agent of human disease that affects millions of individuals worldwide. Historically, our understanding of the mechanisms that contribute to its pathogenesis has been limited. However, the recent development of powerful genetic tools for manipulating Chlamydia has resulted in significant gains in our ability to dissect its virulence mechanisms. These tools have overcome several barriers for manipulating intracellular pathogens and are amenable for the routine genetic engineering of Chlamydia. Here, we provide several detailed protocols for performing genetic analysis in Chlamydia trachomatis allowing investigators to elucidate how this obligate intracellular pathogen causes disease.

Full Text

Duke Authors

Cited Authors

  • KÄ™dzior, M; Bastidas, RJ

Published Date

  • 2019

Volume / Issue

  • 2042 /

Start / End Page

  • 185 - 204

PubMed ID

  • 31385277

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-9694-0_13