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Immunophenotypic and diagnostic characterization of angioimmunoblastic T-cell lymphoma by advanced flow cytometric technology.

Publication ,  Journal Article
Loghavi, S; Wang, SA; Medeiros, LJ; Jorgensen, JL; Li, X; Xu-Monette, ZY; Miranda, RN; Young, KH
Published in: Leukemia & lymphoma
December 2016

Angioimmunoblastic T-cell lymphoma (AITL) often shows systemic symptoms related to immune dysregulation and cytokine production. Biopsy usually harbors few malignant cells in an abundant reactive background, which can be diagnostically challenging in cases with small biopsies. This study was performed to assess the value of flow cytometry (FC) and to determine the immunophenotypic alterations in 155 samples from 38 patients with AITL. FC detected an aberrant T-cell population in 97 of 155 samples that represented 0.5-90% of lymphocytes. Blood was involved in 11 of 16 patients. The most frequent immunophenotypic aberrancies included loss of CD3; altered T-cell receptor expression and aberrant CD10 expression. Altered CD3 expression was more frequently seen in peripheral blood (PB) and bone marrow (BM), whereas aberrant CD10 expression was more common in lymph node (LN). AITL cells often exhibit abnormal CD4+ immunophenotype with diminished or absent CD3 and variable CD10 expression. Multiparameter FC is an effective tool for supporting the diagnosis of AITL in any fluid and various tissue specimens types.

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Published In

Leukemia & lymphoma

DOI

EISSN

1029-2403

ISSN

1042-8194

Publication Date

December 2016

Volume

57

Issue

12

Start / End Page

2804 / 2812

Related Subject Headings

  • Receptors, Antigen, T-Cell
  • Neoplasm Staging
  • Middle Aged
  • Male
  • Lymphoma, T-Cell
  • Lymph Nodes
  • Immunophenotyping
  • Immunology
  • Humans
  • Gene Rearrangement, T-Lymphocyte
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Loghavi, S., Wang, S. A., Medeiros, L. J., Jorgensen, J. L., Li, X., Xu-Monette, Z. Y., … Young, K. H. (2016). Immunophenotypic and diagnostic characterization of angioimmunoblastic T-cell lymphoma by advanced flow cytometric technology. Leukemia & Lymphoma, 57(12), 2804–2812. https://doi.org/10.3109/10428194.2016.1170827
Loghavi, Sanam, Sa A. Wang, L Jeffrey Medeiros, Jeffrey L. Jorgensen, Xin Li, Zijun Y. Xu-Monette, Roberto N. Miranda, and Ken H. Young. “Immunophenotypic and diagnostic characterization of angioimmunoblastic T-cell lymphoma by advanced flow cytometric technology.Leukemia & Lymphoma 57, no. 12 (December 2016): 2804–12. https://doi.org/10.3109/10428194.2016.1170827.
Loghavi S, Wang SA, Medeiros LJ, Jorgensen JL, Li X, Xu-Monette ZY, et al. Immunophenotypic and diagnostic characterization of angioimmunoblastic T-cell lymphoma by advanced flow cytometric technology. Leukemia & lymphoma. 2016 Dec;57(12):2804–12.
Loghavi, Sanam, et al. “Immunophenotypic and diagnostic characterization of angioimmunoblastic T-cell lymphoma by advanced flow cytometric technology.Leukemia & Lymphoma, vol. 57, no. 12, Dec. 2016, pp. 2804–12. Epmc, doi:10.3109/10428194.2016.1170827.
Loghavi S, Wang SA, Medeiros LJ, Jorgensen JL, Li X, Xu-Monette ZY, Miranda RN, Young KH. Immunophenotypic and diagnostic characterization of angioimmunoblastic T-cell lymphoma by advanced flow cytometric technology. Leukemia & lymphoma. 2016 Dec;57(12):2804–2812.

Published In

Leukemia & lymphoma

DOI

EISSN

1029-2403

ISSN

1042-8194

Publication Date

December 2016

Volume

57

Issue

12

Start / End Page

2804 / 2812

Related Subject Headings

  • Receptors, Antigen, T-Cell
  • Neoplasm Staging
  • Middle Aged
  • Male
  • Lymphoma, T-Cell
  • Lymph Nodes
  • Immunophenotyping
  • Immunology
  • Humans
  • Gene Rearrangement, T-Lymphocyte