Scholars@Duke

An intravital window to image the colon in real time.

Publication ,  Journal Article
Rakhilin, N; Garrett, A; Eom, C-Y; Chavez, KR; Small, DM; Daniel, AR; Kaelberer, MM; Mejooli, MA; Huang, Q; Ding, S; Kirsch, DG; Bohórquez, DV ...
Published in: Nat Commun
December 11, 2019
Published version (DOI) Link to item

Intravital microscopy is a powerful technique to observe dynamic processes with single-cell resolution in live animals. No intravital window has been developed for imaging the colon due to its anatomic location and motility, although the colon is a key organ where the majority of microbiota reside and common diseases such as inflammatory bowel disease, functional gastrointestinal disorders, and colon cancer occur. Here we describe an intravital murine colonic window with a stabilizing ferromagnetic scaffold for chronic imaging, minimizing motion artifacts while maximizing long-term survival by preventing colonic obstruction. Using this setup, we image fluorescently-labeled stem cells, bacteria, and immune cells in live animal colons. Furthermore, we image nerve activity via calcium imaging in real time to demonstrate that electrical sacral nerve stimulation can activate colonic enteric neurons. The simple implantable apparatus enables visualization of live processes in the colon, which will open the window to a broad range of studies.

Duke Scholars

Melanie Maya Kaelberer
Author Melanie Maya Kaelberer Medicine, Gastroenterology
David Guy Kirsch
Author David Guy Kirsch Radiation Oncology
Diego V. Bohorquez
Author Diego V. Bohorquez Medicine, Gastroenterology
Xiling Shen
Author Xiling Shen Pathology
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Published In

Nat Commun

DOI

10.1038/s41467-019-13699-w

EISSN

2041-1723

Publication Date

December 11, 2019

Volume

10

Issue

1

Start / End Page

5647

Location

England

Related Subject Headings

  • Stem Cells
  • Optical Imaging
  • Mice, Inbred C57BL
  • Mice
  • Intravital Microscopy
  • In Vitro Techniques
  • Fluorescent Dyes
  • Colon
  • Cell Movement
  • Animals
 

Citation

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Rakhilin, N., Garrett, A., Eom, C.-Y., Chavez, K. R., Small, D. M., Daniel, A. R., … Shen, X. (2019). An intravital window to image the colon in real time. Nat Commun, 10(1), 5647. https://doi.org/10.1038/s41467-019-13699-w
Rakhilin, Nikolai, Aliesha Garrett, Chi-Yong Eom, Katherine Ramos Chavez, David M. Small, Andrea R. Daniel, Melanie M. Kaelberer, et al. “An intravital window to image the colon in real time.” Nat Commun 10, no. 1 (December 11, 2019): 5647. https://doi.org/10.1038/s41467-019-13699-w.
Rakhilin N, Garrett A, Eom C-Y, Chavez KR, Small DM, Daniel AR, et al. An intravital window to image the colon in real time. Nat Commun. 2019 Dec 11;10(1):5647.
Rakhilin, Nikolai, et al. “An intravital window to image the colon in real time.” Nat Commun, vol. 10, no. 1, Dec. 2019, p. 5647. Pubmed, doi:10.1038/s41467-019-13699-w.
Rakhilin N, Garrett A, Eom C-Y, Chavez KR, Small DM, Daniel AR, Kaelberer MM, Mejooli MA, Huang Q, Ding S, Kirsch DG, Bohórquez DV, Nishimura N, Barth BB, Shen X. An intravital window to image the colon in real time. Nat Commun. 2019 Dec 11;10(1):5647.

Published In

Nat Commun

DOI

10.1038/s41467-019-13699-w

EISSN

2041-1723

Publication Date

December 11, 2019

Volume

10

Issue

1

Start / End Page

5647

Location

England

Related Subject Headings

  • Stem Cells
  • Optical Imaging
  • Mice, Inbred C57BL
  • Mice
  • Intravital Microscopy
  • In Vitro Techniques
  • Fluorescent Dyes
  • Colon
  • Cell Movement
  • Animals