Working strokes by single molecules of the kinesin-related microtubule motor ncd.
Publication
, Journal Article
deCastro, MJ; Fondecave, RM; Clarke, LA; Schmidt, CF; Stewart, RJ
Published in: Nature cell biology
October 2000
The ncd protein is a dimeric, ATP-powered motor that belongs to the kinesin family of microtubule motor proteins. Here we resolve single mechanochemical cycles of recombinant, dimeric, full-length ncd, using optical-tweezers-based instrumentation and a three-bead, suspended-microtubule assay. Under conditions of limiting ATP, isolated and transient microtubule-binding events exhibit exponentially distributed and ATP-concentration-dependent lifetimes. These events do not involve consecutive steps along the microtubule, quantitatively confirming that ncd is non-processive. At low loads, a single motor molecule produces ATP-triggered working strokes of about 9 nm, which occur at the ends of binding events.
Duke Scholars
Published In
Nature cell biology
DOI
EISSN
1476-4679
ISSN
1465-7392
Publication Date
October 2000
Volume
2
Issue
10
Start / End Page
724 / 729
Related Subject Headings
- Optics and Photonics
- Movement
- Molecular Motor Proteins
- Models, Theoretical
- Microtubules
- Kinesins
- Drosophila Proteins
- Developmental Biology
- Biomechanical Phenomena
- Adenosine Triphosphate
Citation
APA
Chicago
ICMJE
MLA
NLM
deCastro, M. J., Fondecave, R. M., Clarke, L. A., Schmidt, C. F., & Stewart, R. J. (2000). Working strokes by single molecules of the kinesin-related microtubule motor ncd. Nature Cell Biology, 2(10), 724–729. https://doi.org/10.1038/35036357
deCastro, M. J., R. M. Fondecave, L. A. Clarke, C. F. Schmidt, and R. J. Stewart. “Working strokes by single molecules of the kinesin-related microtubule motor ncd.” Nature Cell Biology 2, no. 10 (October 2000): 724–29. https://doi.org/10.1038/35036357.
deCastro MJ, Fondecave RM, Clarke LA, Schmidt CF, Stewart RJ. Working strokes by single molecules of the kinesin-related microtubule motor ncd. Nature cell biology. 2000 Oct;2(10):724–9.
deCastro, M. J., et al. “Working strokes by single molecules of the kinesin-related microtubule motor ncd.” Nature Cell Biology, vol. 2, no. 10, Oct. 2000, pp. 724–29. Epmc, doi:10.1038/35036357.
deCastro MJ, Fondecave RM, Clarke LA, Schmidt CF, Stewart RJ. Working strokes by single molecules of the kinesin-related microtubule motor ncd. Nature cell biology. 2000 Oct;2(10):724–729.
Published In
Nature cell biology
DOI
EISSN
1476-4679
ISSN
1465-7392
Publication Date
October 2000
Volume
2
Issue
10
Start / End Page
724 / 729
Related Subject Headings
- Optics and Photonics
- Movement
- Molecular Motor Proteins
- Models, Theoretical
- Microtubules
- Kinesins
- Drosophila Proteins
- Developmental Biology
- Biomechanical Phenomena
- Adenosine Triphosphate