A lytic polysaccharide monooxygenase-like protein functions in fungal copper import and meningitis.
Journal Article (Journal Article)
Infection by the fungal pathogen Cryptococcus neoformans causes lethal meningitis, primarily in immune-compromised individuals. Colonization of the brain by C. neoformans is dependent on copper (Cu) acquisition from the host, which drives critical virulence mechanisms. While C. neoformans Cu+ import and virulence are dependent on the Ctr1 and Ctr4 proteins, little is known concerning extracellular Cu ligands that participate in this process. We identified a C. neoformans gene, BIM1, that is strongly induced during Cu limitation and which encodes a protein related to lytic polysaccharide monooxygenases (LPMOs). Surprisingly, bim1 mutants are Cu deficient, and Bim1 function in Cu accumulation depends on Cu2+ coordination and cell-surface association via a glycophosphatidyl inositol anchor. Bim1 participates in Cu uptake in concert with Ctr1 and expression of this pathway drives brain colonization in mouse infection models. These studies demonstrate a role for LPMO-like proteins as a critical factor for Cu acquisition in fungal meningitis.
Full Text
Duke Authors
Cited Authors
- Garcia-Santamarina, S; Probst, C; Festa, RA; Ding, C; Smith, AD; Conklin, SE; Brander, S; Kinch, LN; Grishin, NV; Franz, KJ; Riggs-Gelasco, P; Lo Leggio, L; Johansen, KS; Thiele, DJ
Published Date
- March 2020
Published In
Volume / Issue
- 16 / 3
Start / End Page
- 337 - 344
PubMed ID
- 31932719
Pubmed Central ID
- PMC7036007
Electronic International Standard Serial Number (EISSN)
- 1552-4469
International Standard Serial Number (ISSN)
- 1552-4450
Digital Object Identifier (DOI)
- 10.1038/s41589-019-0437-9
Language
- eng