Over-expression of fibroblast growth factor receptor 3 in human hepatocellular carcinoma.

Journal Article (Journal Article)

AIM: To describe the significant over-expression of fibroblast growth factor receptor 3 (FGFR3), which is a signal transduction and cell proliferation related gene in hepatocellular carcinoma (HCC). METHODS: Following DNA microarray, Northern blot and quantitative real-time PCR were employed to confirm FGFR3 expression difference in HCC tissues and surrounding non-neoplastic liver tissue. FGFR3 expression levels were further determined by immunohistochemical study in 43 cases of HCC. RESULTS: Northern blot results showed the significant over-expression of FGFR3 in HCC tissues, which was consistent with that from DNA microarray. Quantitative real-time PCR demonstrated that the mean ratio of FGFR3 mRNA to glyceraldehyde-3-phosphate dehydrogenase (GADPH) mRNA in HCC tissue was 0.250, whereas the ratio in non-neoplastic liver tissue was 0.014. Statistical analyses of 43 cases of HCC revealed that HCC scored higher than the matched non-neoplastic liver tissues. Examination of clinicopathological features revealed a strong correlation of over-expression of FGFR3 with poor tumor differentiation and high nuclear grade. CONCLUSION: Over-expression of FGFR3 may play an important role in liver carcinogenesis. FGFR3 may be an ideal candidate as a molecular marker in the diagnosis of HCC and a potential therapeutic target.

Full Text

Duke Authors

Cited Authors

  • Qiu, W-H; Zhou, B-S; Chu, P-G; Chen, W-G; Chung, C; Shih, J; Hwu, P; Yeh, C; Lopez, R; Yen, Y

Published Date

  • September 14, 2005

Published In

Volume / Issue

  • 11 / 34

Start / End Page

  • 5266 - 5272

PubMed ID

  • 16149130

Pubmed Central ID

  • 16149130

International Standard Serial Number (ISSN)

  • 1007-9327

Digital Object Identifier (DOI)

  • 10.3748/wjg.v11.i34.5266


  • eng

Conference Location

  • United States