Carbon Monoxide and Exercise Prevents Diet-Induced Obesity and Metabolic Dysregulation Without Affecting Bone.

Journal Article (Journal Article)


Carbon monoxide (CO) may counteract obesity and metabolic dysfunction in rodents consuming high-fat diets, but the skeletal effects are not understood. This study investigated whether low-dose inhaled CO (250 ppm) with or without moderate intensity aerobic exercise (3 h/wk) would limit diet-induced obesity and metabolic dysregulation and preserve bone health.


Obesity-resistant (OR) rats served as controls, and obesity-prone (OP) rats were randomized to sedentary, sedentary plus CO, exercise, or CO plus exercise. For 10 weeks, OP rats consumed a high-fat, high-sucrose diet, whereas OR rats consumed a low-fat control diet. Measurements included indicators of obesity and metabolism, bone turnover markers, femoral geometry and microarchitecture, bone mechanical properties, and tibial morphometry.


A high-fat, high-sucrose diet led to obesity, hyperinsulinemia, and hyperleptinemia, without impacting bone. CO alone led only to a modest reduction in weight gain. Exercise attenuated weight gain and improved the metabolic profile; however, bone fragility increased. Combined CO and exercise led to body mass reduction and a metabolic state similar to control OR rats and prevented the exercise-induced increase in bone fragility.


CO and aerobic exercise training prevent obesity and metabolic sequelae of nutrient excess while stabilizing bone physiology.

Full Text

Duke Authors

Cited Authors

  • Gasier, HG; Yu, T; Swift, JM; Metzger, CE; McNerny, EM; Swallow, EA; Piantadosi, CA; Allen, MR

Published Date

  • May 2020

Published In

Volume / Issue

  • 28 / 5

Start / End Page

  • 924 - 931

PubMed ID

  • 32237119

Electronic International Standard Serial Number (EISSN)

  • 1930-739X

International Standard Serial Number (ISSN)

  • 1930-7381

Digital Object Identifier (DOI)

  • 10.1002/oby.22768


  • eng