IL-27 signaling activates skin cells to induce innate antiviral proteins and protects against Zika virus infection.

Journal Article (Journal Article)

In the skin, antiviral proteins and other immune molecules serve as the first line of innate antiviral defense. Here, we identify and characterize the induction of cutaneous innate antiviral proteins in response to IL-27 and its functional role during cutaneous defense against Zika virus infection. Transcriptional and phenotypic profiling of epidermal keratinocytes treated with IL-27 demonstrated activation of antiviral proteins OAS1, OAS2, OASL, and MX1 in the skin of both mice and humans. IL-27-mediated antiviral protein induction was found to occur in a STAT1- and IRF3-dependent but STAT2-independent manner. Moreover, using IL27ra mice, we demonstrate a significant role for IL-27 in inhibiting Zika virus morbidity and mortality following cutaneous, but not intravenous, inoculation. Together, our results demonstrate a critical and previously unrecognized role for IL-27 in cutaneous innate antiviral immunity against Zika virus.

Full Text

Duke Authors

Cited Authors

  • Kwock, JT; Handfield, C; Suwanpradid, J; Hoang, P; McFadden, MJ; Labagnara, KF; Floyd, L; Shannon, J; Uppala, R; Sarkar, MK; Gudjonsson, JE; Corcoran, DL; Lazear, HM; Sempowski, G; Horner, SM; MacLeod, AS

Published Date

  • April 2020

Published In

Volume / Issue

  • 6 / 14

Start / End Page

  • eaay3245 -

PubMed ID

  • 32270034

Pubmed Central ID

  • PMC7112749

Electronic International Standard Serial Number (EISSN)

  • 2375-2548

Digital Object Identifier (DOI)

  • 10.1126/sciadv.aay3245

Language

  • eng

Conference Location

  • United States