Lack of Targetable FGFR2 Fusions in Endemic Fluke-Associated Cholangiocarcinoma.

Journal Article (Journal Article)

PURPOSE: Cholangiocarcinoma (CCA) remains a disease with poor prognosis and limited therapeutic options. Identification of driver genetic alterations may lead to the discovery of more effective targeted therapies. CCAs harboring FGFR2 fusions have recently demonstrated promising responses to FGFR inhibitors, highlighting their potential relevance as predictive biomarkers. CCA incidence is high in the northeast of Thailand and its neighboring countries because of chronic infection with the liver fluke Opisthorchis viverrini (Ov). However, there are currently no available data on the prevalence of FGFR alterations in fluke-associated CCA in endemic countries. MATERIALS AND METHODS: In this study, we performed anchored multiplex polymerase chain reaction target enrichment RNA sequencing of FGFR1-3, validated by fluorescence in situ hybridization and Sanger sequencing, in 121 Ov-associated and 95 non-Ov-associated CCA tumors. RESULTS: Compared with non-fluke-associated CCA (11/95; 11.6%), FGFR2 fusions were significantly less common in fluke-associated CCA (1/121; 0.8%; P = .0006). All FGFR fusions were detected exclusively in intrahepatic CCAs and were mutually exclusive with KRAS/ERBB2/BRAF/FGFR mutations, pointing to their potential roles as oncogenic drivers. CONCLUSION: FGFR2 fusions are rare in fluke-associated CCA, underscoring how distinct etiologies may affect molecular landscapes in tumors and highlighting the need to discover other actionable genomic alterations in endemic fluke-associated CCA.

Full Text

Duke Authors

Cited Authors

  • Kongpetch, S; Jusakul, A; Lim, JQ; Ng, CCY; Chan, JY; Rajasegaran, V; Lim, TH; Lim, KH; Choo, SP; Dima, S; Popescu, I; Duda, DG; Kukongviriyapan, V; Khuntikeo, N; Pairojkul, C; Rozen, SG; Tan, P; Teh, BT

Published Date

  • April 2020

Published In

Volume / Issue

  • 6 /

Start / End Page

  • 628 - 638

PubMed ID

  • 32315234

Pubmed Central ID

  • PMC7193781

Electronic International Standard Serial Number (EISSN)

  • 2687-8941

Digital Object Identifier (DOI)

  • 10.1200/GO.20.00030

Language

  • eng

Conference Location

  • United States