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CDK-Regulated Phase Separation Seeded by Histone Genes Ensures Precise Growth and Function of Histone Locus Bodies.

Publication ,  Journal Article
Hur, W; Kemp, JP; Tarzia, M; Deneke, VE; Marzluff, WF; Duronio, RJ; Di Talia, S
Published in: Dev Cell
August 10, 2020

Many membraneless organelles form through liquid-liquid phase separation, but how their size is controlled and whether size is linked to function remain poorly understood. The histone locus body (HLB) is an evolutionarily conserved nuclear body that regulates the transcription and processing of histone mRNAs. Here, we show that Drosophila HLBs form through phase separation. During embryogenesis, the size of HLBs is controlled in a precise and dynamic manner that is dependent on the cell cycle and zygotic histone gene activation. Control of HLB growth is achieved by a mechanism integrating nascent mRNAs at the histone locus, which facilitates phase separation, and the nuclear concentration of the scaffold protein multi-sex combs (Mxc), which is controlled by the activity of cyclin-dependent kinases. Reduced Cdk2 activity results in smaller HLBs and the appearance of nascent, misprocessed histone mRNAs. Thus, our experiments identify a mechanism linking nuclear body growth and size with gene expression.

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Published In

Dev Cell

DOI

EISSN

1878-1551

Publication Date

August 10, 2020

Volume

54

Issue

3

Start / End Page

379 / 394.e6

Location

United States

Related Subject Headings

  • Transcriptional Activation
  • RNA, Messenger
  • Histones
  • Embryonic Development
  • Drosophila melanogaster
  • Drosophila Proteins
  • Drosophila
  • Developmental Biology
  • Cell Nucleus
  • Cell Cycle Proteins
 

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Hur, W., Kemp, J. P., Tarzia, M., Deneke, V. E., Marzluff, W. F., Duronio, R. J., & Di Talia, S. (2020). CDK-Regulated Phase Separation Seeded by Histone Genes Ensures Precise Growth and Function of Histone Locus Bodies. Dev Cell, 54(3), 379-394.e6. https://doi.org/10.1016/j.devcel.2020.06.003
Hur, Woonyung, James P. Kemp, Marco Tarzia, Victoria E. Deneke, William F. Marzluff, Robert J. Duronio, and Stefano Di Talia. “CDK-Regulated Phase Separation Seeded by Histone Genes Ensures Precise Growth and Function of Histone Locus Bodies.Dev Cell 54, no. 3 (August 10, 2020): 379-394.e6. https://doi.org/10.1016/j.devcel.2020.06.003.
Hur W, Kemp JP, Tarzia M, Deneke VE, Marzluff WF, Duronio RJ, et al. CDK-Regulated Phase Separation Seeded by Histone Genes Ensures Precise Growth and Function of Histone Locus Bodies. Dev Cell. 2020 Aug 10;54(3):379-394.e6.
Hur, Woonyung, et al. “CDK-Regulated Phase Separation Seeded by Histone Genes Ensures Precise Growth and Function of Histone Locus Bodies.Dev Cell, vol. 54, no. 3, Aug. 2020, pp. 379-394.e6. Pubmed, doi:10.1016/j.devcel.2020.06.003.
Hur W, Kemp JP, Tarzia M, Deneke VE, Marzluff WF, Duronio RJ, Di Talia S. CDK-Regulated Phase Separation Seeded by Histone Genes Ensures Precise Growth and Function of Histone Locus Bodies. Dev Cell. 2020 Aug 10;54(3):379-394.e6.
Journal cover image

Published In

Dev Cell

DOI

EISSN

1878-1551

Publication Date

August 10, 2020

Volume

54

Issue

3

Start / End Page

379 / 394.e6

Location

United States

Related Subject Headings

  • Transcriptional Activation
  • RNA, Messenger
  • Histones
  • Embryonic Development
  • Drosophila melanogaster
  • Drosophila Proteins
  • Drosophila
  • Developmental Biology
  • Cell Nucleus
  • Cell Cycle Proteins