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Modified glycan models of pig-to-human xenotransplantation do not enhance the human-anti-pig T cell response.

Publication ,  Journal Article
Butler, JR; Wang, Z-Y; Martens, GR; Ladowski, JM; Li, P; Tector, M; Tector, AJ
Published in: Transplant immunology
March 2016

Genetically modified porcine models of pig-to-human xenotransplantation offer the most immediate answer to a growing shortage of available solid organs. Recently a modified porcine glycan model has been discovered that reduces human antibody binding to levels comparable with allograft standards. As this background provides an answer to the problem of acute humoral xenograft rejection (AHXR), it is important to consider the impact these modifications have on measures of cell-mediated rejection. The objective of this study was to examine the impact of currently relevant glycan knockout models of pig-to-human xenotransplantation in a lymphocyte proliferation assay. To accomplish these goals, genetically modified pigs were created through CRISPR/Cas9-directed silencing of the GGTA1, and CMAH genes. Peripheral blood mononuclear cells (PBMCs) and spleen cells were obtained from these animals and used as a source of stimulation for human responders in one-way mixed lymphocyte reactions. The response was tested in the presence and absence of clinically available immunomodifiers.Clinically relevant glycan knockout models of pig-to-human xenotransplantation do not enhance the human-anti-pig cellular response. Currently available and conventional immunosuppression has the capacity to mediate the human xenogeneic T cell response to these knockout cells.

Duke Scholars

Published In

Transplant immunology

DOI

EISSN

1878-5492

ISSN

0966-3274

Publication Date

March 2016

Volume

35

Start / End Page

47 / 51

Related Subject Headings

  • Swine
  • Surgery
  • Polysaccharides
  • Organ Transplantation
  • Models, Immunological
  • Mixed Function Oxygenases
  • Lymphocytes
  • Humans
  • Heterografts
  • Galactosyltransferases
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Butler, J. R., Wang, Z.-Y., Martens, G. R., Ladowski, J. M., Li, P., Tector, M., & Tector, A. J. (2016). Modified glycan models of pig-to-human xenotransplantation do not enhance the human-anti-pig T cell response. Transplant Immunology, 35, 47–51. https://doi.org/10.1016/j.trim.2016.02.001
Butler, James R., Zheng-Yu Wang, Gregory R. Martens, Joseph M. Ladowski, Ping Li, Matthew Tector, and A Joseph Tector. “Modified glycan models of pig-to-human xenotransplantation do not enhance the human-anti-pig T cell response.Transplant Immunology 35 (March 2016): 47–51. https://doi.org/10.1016/j.trim.2016.02.001.
Butler JR, Wang Z-Y, Martens GR, Ladowski JM, Li P, Tector M, et al. Modified glycan models of pig-to-human xenotransplantation do not enhance the human-anti-pig T cell response. Transplant immunology. 2016 Mar;35:47–51.
Butler, James R., et al. “Modified glycan models of pig-to-human xenotransplantation do not enhance the human-anti-pig T cell response.Transplant Immunology, vol. 35, Mar. 2016, pp. 47–51. Epmc, doi:10.1016/j.trim.2016.02.001.
Butler JR, Wang Z-Y, Martens GR, Ladowski JM, Li P, Tector M, Tector AJ. Modified glycan models of pig-to-human xenotransplantation do not enhance the human-anti-pig T cell response. Transplant immunology. 2016 Mar;35:47–51.
Journal cover image

Published In

Transplant immunology

DOI

EISSN

1878-5492

ISSN

0966-3274

Publication Date

March 2016

Volume

35

Start / End Page

47 / 51

Related Subject Headings

  • Swine
  • Surgery
  • Polysaccharides
  • Organ Transplantation
  • Models, Immunological
  • Mixed Function Oxygenases
  • Lymphocytes
  • Humans
  • Heterografts
  • Galactosyltransferases