Controlled Lengthwise Assembly of Helical Peptide Nanofibers to Modulate CD8+ T-Cell Responses.

Journal Article (Journal Article)

Peptide nanofibers are useful for many biological applications, including immunotherapy, tissue engineering, and drug delivery. The robust lengthwise assembly of these peptides into nanofibers is typically difficult to control, resulting in polydisperse fiber lengths and an incomplete understanding of how nanofiber length affects biological responses. Here, rationally designed capping peptides control the length of helical peptide nanofibers with unique precision. These designed peptides bind the tips of elongated nanofibers to shorten and narrow their length distributions. Demonstrating their use as immunotherapies, capped nanofibers are preferentially cross-presented by dendritic cells compared to uncapped nanofibers. Due to increased cross-presentation, these capped nanofibers trigger stronger CD8+ T-cell responses in mice than uncapped nanofibers. This strategy illustrates a means for controlling the length of supramolecular peptide nanofibers to modulate their immunogenicity in the context of immunotherapies.

Full Text

Duke Authors

Cited Authors

  • Fries, CN; Wu, Y; Kelly, SH; Wolf, M; Votaw, NL; Zauscher, S; Collier, JH

Published Date

  • October 2020

Published In

Volume / Issue

  • 32 / 39

Start / End Page

  • e2003310 -

PubMed ID

  • 32820582

Pubmed Central ID

  • PMC7719602

Electronic International Standard Serial Number (EISSN)

  • 1521-4095

International Standard Serial Number (ISSN)

  • 0935-9648

Digital Object Identifier (DOI)

  • 10.1002/adma.202003310

Language

  • eng