A Genetic Cardiomyocyte Ablation Model for the Study of Heart Regeneration in Zebrafish.

Journal Article (Journal Article)

Adult zebrafish possess an elevated cardiac regenerative capacity as compared with adult mammals. In the past two decades, zebrafish have provided a key model system for studying the cellular and molecular mechanisms of innate heart regeneration. The ease of genetic manipulation in zebrafish has enabled the establishment of a genetic ablation injury model in which over 60% of cardiomyocytes can be depleted, eliciting signs of heart failure. After this severe injury, adult zebrafish efficiently regenerate lost cardiomyocytes and reverse heart failure. In this chapter, we describe the methods for inducing genetic cardiomyocyte ablation in adult zebrafish, assessing cardiomyocyte proliferation, and histologically analyzing regeneration after injury.

Full Text

Duke Authors

Cited Authors

  • Sun, F; Shoffner, AR; Poss, KD

Published Date

  • 2021

Published In

Volume / Issue

  • 2158 /

Start / End Page

  • 71 - 80

PubMed ID

  • 32857367

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-0716-0668-1_7

Language

  • eng

Conference Location

  • United States