Skip to main content

Multiple Nod-like receptors activate caspase 1 during Listeria monocytogenes infection.

Publication ,  Journal Article
Warren, SE; Mao, DP; Rodriguez, AE; Miao, EA; Aderem, A
Published in: J Immunol
June 1, 2008

Listeria monocytogenes escapes from the phagosome of macrophages and replicates within the cytosolic compartment. The macrophage responds to L. monocytogenes through detection pathways located on the cell surface (TLRs) and within the cytosol (Nod-like receptors) to promote inflammatory processes aimed at clearing the pathogen. Cytosolic L. monocytogenes activates caspase 1, resulting in post-translational processing of the cytokines IL-1beta and IL-18 as well as caspase 1-dependent cell death (pyroptosis). We demonstrate that the presence of L. monocytogenes within the cytosolic compartment induces caspase 1 activation through multiple Nod-like receptors, including Ipaf and Nalp3. Flagellin expression by cytosolic L. monocytogenes was detected through Ipaf in a dose-dependent manner. Concordantly, detection of flagellin promoted bacterial clearance in a murine infection model. Finally, we provide evidence that suggests cytosolic L. monocytogenes activates caspase 1 through a third pathway, which signals through the adaptor protein ASC. Thus, L. monocytogenes activates caspase 1 in macrophages via multiple pathways, all of which detect the presence of bacteria within the cytosol.

Duke Scholars

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

June 1, 2008

Volume

180

Issue

11

Start / End Page

7558 / 7564

Location

United States

Related Subject Headings

  • Toll-Like Receptors
  • Phagosomes
  • NLR Family, Pyrin Domain-Containing 3 Protein
  • Mice, Mutant Strains
  • Mice, Inbred C57BL
  • Mice
  • Macrophages
  • Listeriosis
  • Listeria monocytogenes
  • Interleukin-1beta
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Warren, S. E., Mao, D. P., Rodriguez, A. E., Miao, E. A., & Aderem, A. (2008). Multiple Nod-like receptors activate caspase 1 during Listeria monocytogenes infection. J Immunol, 180(11), 7558–7564. https://doi.org/10.4049/jimmunol.180.11.7558
Warren, Sarah E., Dat P. Mao, April E. Rodriguez, Edward A. Miao, and Alan Aderem. “Multiple Nod-like receptors activate caspase 1 during Listeria monocytogenes infection.J Immunol 180, no. 11 (June 1, 2008): 7558–64. https://doi.org/10.4049/jimmunol.180.11.7558.
Warren SE, Mao DP, Rodriguez AE, Miao EA, Aderem A. Multiple Nod-like receptors activate caspase 1 during Listeria monocytogenes infection. J Immunol. 2008 Jun 1;180(11):7558–64.
Warren, Sarah E., et al. “Multiple Nod-like receptors activate caspase 1 during Listeria monocytogenes infection.J Immunol, vol. 180, no. 11, June 2008, pp. 7558–64. Pubmed, doi:10.4049/jimmunol.180.11.7558.
Warren SE, Mao DP, Rodriguez AE, Miao EA, Aderem A. Multiple Nod-like receptors activate caspase 1 during Listeria monocytogenes infection. J Immunol. 2008 Jun 1;180(11):7558–7564.

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

June 1, 2008

Volume

180

Issue

11

Start / End Page

7558 / 7564

Location

United States

Related Subject Headings

  • Toll-Like Receptors
  • Phagosomes
  • NLR Family, Pyrin Domain-Containing 3 Protein
  • Mice, Mutant Strains
  • Mice, Inbred C57BL
  • Mice
  • Macrophages
  • Listeriosis
  • Listeria monocytogenes
  • Interleukin-1beta