Spatial scanning of a sample with two-dimensional angle-resolved low-coherence interferometry for analysis of anisotropic scatterers.

Published

Journal Article

Angle-resolved low-coherence interferometry (a/LCI) measures depth-resolved angular scattering for cell nuclear morphology analysis. 2D a/LCI, developed to collect across two scattering planes, is currently limited by the lack of spatial scanning. Here we demonstrate 2D a/LCI scanning across a three-dimensional volume using an image rotation scheme and a scanning mirror. Validation using various optical phantoms demonstrated excellent scatterer size determination over a 7.5 mm linear range, for a total accessible area of ∼44 mm2. Measurements from anisotropic scatterers allowed accurate determination of sizes and computation of aspect ratios. This scanning system will facilitate analysis of scatterer structure across wider tissue areas.

Full Text

Duke Authors

Cited Authors

  • Song, G; Steelman, ZA; Kendall, W; Park, HS; Wax, A

Published Date

  • August 2020

Published In

Volume / Issue

  • 11 / 8

Start / End Page

  • 4419 - 4430

PubMed ID

  • 32923053

Pubmed Central ID

  • 32923053

Electronic International Standard Serial Number (EISSN)

  • 2156-7085

International Standard Serial Number (ISSN)

  • 2156-7085

Digital Object Identifier (DOI)

  • 10.1364/boe.398052

Language

  • eng