Rapid phosphoproteomic and transcriptomic changes in the rhizobia-legume symbiosis.

Journal Article (Journal Article)

Symbiotic associations between legumes and rhizobia usually commence with the perception of bacterial lipochitooligosaccharides, known as Nod factors (NF), which triggers rapid cellular and molecular responses in host plants. We report here deep untargeted tandem mass spectrometry-based measurements of rapid NF-induced changes in the phosphorylation status of 13,506 phosphosites in 7739 proteins from the model legume Medicago truncatula. To place these phosphorylation changes within a biological context, quantitative phosphoproteomic and RNA measurements in wild-type plants were compared with those observed in mutants, one defective in NF perception (nfp) and one defective in downstream signal transduction events (dmi3). Our study quantified the early phosphorylation and transcription dynamics that are specifically associated with NF-signaling, confirmed a dmi3-mediated feedback loop in the pathway, and suggested "cryptic" NF-signaling pathways, some of them being also involved in the response to symbiotic arbuscular mycorrhizal fungi.

Full Text

Duke Authors

Cited Authors

  • Rose, CM; Venkateshwaran, M; Volkening, JD; Grimsrud, PA; Maeda, J; Bailey, DJ; Park, K; Howes-Podoll, M; den Os, D; Yeun, LH; Westphall, MS; Sussman, MR; Ané, J-M; Coon, JJ

Published Date

  • September 2012

Published In

Volume / Issue

  • 11 / 9

Start / End Page

  • 724 - 744

PubMed ID

  • 22683509

Pubmed Central ID

  • PMC3434772

Electronic International Standard Serial Number (EISSN)

  • 1535-9484

Digital Object Identifier (DOI)

  • 10.1074/mcp.M112.019208

Language

  • eng

Conference Location

  • United States