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Rapid phosphoproteomic and transcriptomic changes in the rhizobia-legume symbiosis.

Publication ,  Journal Article
Rose, CM; Venkateshwaran, M; Volkening, JD; Grimsrud, PA; Maeda, J; Bailey, DJ; Park, K; Howes-Podoll, M; den Os, D; Yeun, LH; Westphall, MS ...
Published in: Mol Cell Proteomics
September 2012

Symbiotic associations between legumes and rhizobia usually commence with the perception of bacterial lipochitooligosaccharides, known as Nod factors (NF), which triggers rapid cellular and molecular responses in host plants. We report here deep untargeted tandem mass spectrometry-based measurements of rapid NF-induced changes in the phosphorylation status of 13,506 phosphosites in 7739 proteins from the model legume Medicago truncatula. To place these phosphorylation changes within a biological context, quantitative phosphoproteomic and RNA measurements in wild-type plants were compared with those observed in mutants, one defective in NF perception (nfp) and one defective in downstream signal transduction events (dmi3). Our study quantified the early phosphorylation and transcription dynamics that are specifically associated with NF-signaling, confirmed a dmi3-mediated feedback loop in the pathway, and suggested "cryptic" NF-signaling pathways, some of them being also involved in the response to symbiotic arbuscular mycorrhizal fungi.

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Published In

Mol Cell Proteomics

DOI

EISSN

1535-9484

Publication Date

September 2012

Volume

11

Issue

9

Start / End Page

724 / 744

Location

United States

Related Subject Headings

  • Transcriptome
  • Tandem Mass Spectrometry
  • Symbiosis
  • Sinorhizobium meliloti
  • Signal Transduction
  • Rhizobium
  • Plant Proteins
  • Phosphorylation
  • Phosphoproteins
  • Mycorrhizae
 

Citation

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Rose, C. M., Venkateshwaran, M., Volkening, J. D., Grimsrud, P. A., Maeda, J., Bailey, D. J., … Coon, J. J. (2012). Rapid phosphoproteomic and transcriptomic changes in the rhizobia-legume symbiosis. Mol Cell Proteomics, 11(9), 724–744. https://doi.org/10.1074/mcp.M112.019208
Rose, Christopher M., Muthusubramanian Venkateshwaran, Jeremy D. Volkening, Paul A. Grimsrud, Junko Maeda, Derek J. Bailey, Kwanghyun Park, et al. “Rapid phosphoproteomic and transcriptomic changes in the rhizobia-legume symbiosis.Mol Cell Proteomics 11, no. 9 (September 2012): 724–44. https://doi.org/10.1074/mcp.M112.019208.
Rose CM, Venkateshwaran M, Volkening JD, Grimsrud PA, Maeda J, Bailey DJ, et al. Rapid phosphoproteomic and transcriptomic changes in the rhizobia-legume symbiosis. Mol Cell Proteomics. 2012 Sep;11(9):724–44.
Rose, Christopher M., et al. “Rapid phosphoproteomic and transcriptomic changes in the rhizobia-legume symbiosis.Mol Cell Proteomics, vol. 11, no. 9, Sept. 2012, pp. 724–44. Pubmed, doi:10.1074/mcp.M112.019208.
Rose CM, Venkateshwaran M, Volkening JD, Grimsrud PA, Maeda J, Bailey DJ, Park K, Howes-Podoll M, den Os D, Yeun LH, Westphall MS, Sussman MR, Ané J-M, Coon JJ. Rapid phosphoproteomic and transcriptomic changes in the rhizobia-legume symbiosis. Mol Cell Proteomics. 2012 Sep;11(9):724–744.

Published In

Mol Cell Proteomics

DOI

EISSN

1535-9484

Publication Date

September 2012

Volume

11

Issue

9

Start / End Page

724 / 744

Location

United States

Related Subject Headings

  • Transcriptome
  • Tandem Mass Spectrometry
  • Symbiosis
  • Sinorhizobium meliloti
  • Signal Transduction
  • Rhizobium
  • Plant Proteins
  • Phosphorylation
  • Phosphoproteins
  • Mycorrhizae