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Staphylococcus aureus secretes immunomodulatory RNA and DNA via membrane vesicles.

Publication ,  Journal Article
Rodriguez, BV; Kuehn, MJ
Published in: Sci Rep
October 26, 2020

Bacterial-derived RNA and DNA can function as ligands for intracellular receptor activation and induce downstream signaling to modulate the host response to bacterial infection. The mechanisms underlying the secretion of immunomodulatory RNA and DNA by pathogens such as Staphylococcus aureus and their delivery to intracellular host cell receptors are not well understood. Recently, extracellular membrane vesicle (MV) production has been proposed as a general secretion mechanism that could facilitate the delivery of functional bacterial nucleic acids into host cells. S. aureus produce membrane-bound, spherical, nano-sized, MVs packaged with a select array of bioactive macromolecules and they have been shown to play important roles in bacterial virulence and in immune modulation through the transmission of biologic signals to host cells. Here we show that S. aureus secretes RNA and DNA molecules that are mostly protected from degradation by their association with MVs. Importantly, we demonstrate that MVs can be delivered into cultured macrophage cells and subsequently stimulate a potent IFN-β response in recipient cells via activation of endosomal Toll-like receptors. These findings advance our understanding of the mechanisms by which bacterial nucleic acids traffic extracellularly to trigger the modulation of host immune responses.

Duke Scholars

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Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

October 26, 2020

Volume

10

Issue

1

Start / End Page

18293

Location

England

Related Subject Headings

  • Virulence
  • Toll-Like Receptors
  • Staphylococcus aureus
  • RNA, Bacterial
  • RAW 264.7 Cells
  • Particle Size
  • Mice
  • Macrophages
  • Interferon-gamma
  • Extracellular Vesicles
 

Citation

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Rodriguez, B. V., & Kuehn, M. J. (2020). Staphylococcus aureus secretes immunomodulatory RNA and DNA via membrane vesicles. Sci Rep, 10(1), 18293. https://doi.org/10.1038/s41598-020-75108-3
Rodriguez, Blanca V., and Meta J. Kuehn. “Staphylococcus aureus secretes immunomodulatory RNA and DNA via membrane vesicles.Sci Rep 10, no. 1 (October 26, 2020): 18293. https://doi.org/10.1038/s41598-020-75108-3.
Rodriguez BV, Kuehn MJ. Staphylococcus aureus secretes immunomodulatory RNA and DNA via membrane vesicles. Sci Rep. 2020 Oct 26;10(1):18293.
Rodriguez, Blanca V., and Meta J. Kuehn. “Staphylococcus aureus secretes immunomodulatory RNA and DNA via membrane vesicles.Sci Rep, vol. 10, no. 1, Oct. 2020, p. 18293. Pubmed, doi:10.1038/s41598-020-75108-3.
Rodriguez BV, Kuehn MJ. Staphylococcus aureus secretes immunomodulatory RNA and DNA via membrane vesicles. Sci Rep. 2020 Oct 26;10(1):18293.

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

October 26, 2020

Volume

10

Issue

1

Start / End Page

18293

Location

England

Related Subject Headings

  • Virulence
  • Toll-Like Receptors
  • Staphylococcus aureus
  • RNA, Bacterial
  • RAW 264.7 Cells
  • Particle Size
  • Mice
  • Macrophages
  • Interferon-gamma
  • Extracellular Vesicles