Margarethe Joanna Kuehn
Associate Professor of Biochemistry
Enterotoxigenic E. coli (ETEC) causes traveler's diarrhea and infant mortality in underdeveloped countries, and Pseudomonas aeruginosa is an opportunistic pathogen for immunocompromised patients. Like all gram negative bacteria studied to date, ETEC and P. aeruginosa produce small outer membrane vesicles that can serve as delivery "bombs" to host tissues. Vesicles contain a subset of outer membrane and soluble periplasmic proteins and lipids. In tissues and sera of infected hosts, vesicles have been observed to bud from the pathogen and come in close contact with epithelial cells. Despite their association with disease, the ability of pathogenic bacteria to distribute an arsenal of virulence factors to the host cells via vesicles remains relatively unexplored.
In our lab, we focus on the genetic, biochemical and functional features of bacterial vesicle production. Using a genetic screen, we have identified genes essential in the vesiculation process, we have identified specific proteins that are enriched in vesicles, and we have identified critical molecules that govern the internalization of vesicles into host cells. Using biochemical analysis of purified vesicles from cell-free culture supernatants, we have found that heat-labile enterotoxin, an important virulence factor of ETEC, is exported from the cells bound to the external surface of vesicles. Presented in this context, it is able to mediate the entry of the entire ETEC vesicle into human colorectal tissue culture cells. We have also discovered that the ability of vesicles to bind to specific cell types depends on their strain of origin: for example, P. aeruginosa vesicles produced by a strain that was cultured from the lungs of a patient with Cystic Fibrosis adhered better to lung than to gut epithelial cells, whereas a strain that was isolated from sera showed no such preference for lung cells. The vesicles stimulate epithelial cells and macrophages to elicit a cytokine response that is distinct from that of LPS (a major component of the vesicles) alone.
These studies will provide new insights into the membrane dynamics of gram-negative bacteria and consequently aid in the identification of new therapeutic targets for important human pathogens.
In our lab, we focus on the genetic, biochemical and functional features of bacterial vesicle production. Using a genetic screen, we have identified genes essential in the vesiculation process, we have identified specific proteins that are enriched in vesicles, and we have identified critical molecules that govern the internalization of vesicles into host cells. Using biochemical analysis of purified vesicles from cell-free culture supernatants, we have found that heat-labile enterotoxin, an important virulence factor of ETEC, is exported from the cells bound to the external surface of vesicles. Presented in this context, it is able to mediate the entry of the entire ETEC vesicle into human colorectal tissue culture cells. We have also discovered that the ability of vesicles to bind to specific cell types depends on their strain of origin: for example, P. aeruginosa vesicles produced by a strain that was cultured from the lungs of a patient with Cystic Fibrosis adhered better to lung than to gut epithelial cells, whereas a strain that was isolated from sera showed no such preference for lung cells. The vesicles stimulate epithelial cells and macrophages to elicit a cytokine response that is distinct from that of LPS (a major component of the vesicles) alone.
These studies will provide new insights into the membrane dynamics of gram-negative bacteria and consequently aid in the identification of new therapeutic targets for important human pathogens.
Current Appointments & Affiliations
- Associate Professor of Biochemistry, Biochemistry, Basic Science Departments 2007
- Associate Professor in Molecular Genetics and Microbiology, Molecular Genetics and Microbiology, Basic Science Departments 2007
- Associate Professor of Cell Biology, Cell Biology, Basic Science Departments 2022
- Member of the Duke Cancer Institute, Duke Cancer Institute, Institutes and Centers 1997
Contact Information
- Duke Box 3711, Durham, NC 27710
- Research Drive, 220A Nanaline H Duke, Durham, NC 27710
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kuehn@duke.edu
(919) 684-2545
- Background
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Education, Training, & Certifications
- Howard Hughes Postdoctoral Research Fellow, Biochemistry And Molecular Biolovy, University of California - Berkeley 1994 - 1997
- Postdoctoral Fellow, Molecular Microbiology, Washington University in St. Louis 1993
- Ph.D., Washington University in St. Louis 1993
- B.S., University of Washington 1986
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Previous Appointments & Affiliations
- Assistant Professor of Biochemistry, Biochemistry, Basic Science Departments 1998 - 2006
- Assistant Professor of Molecular Genetics and Microbiology, Molecular Genetics and Microbiology, Basic Science Departments 2002 - 2006
- Assistant Professor of Microbiology, Molecular Genetics and Microbiology, Basic Science Departments 1998 - 2002
- Assistant Research Professor of Biochemistry, Biochemistry, Basic Science Departments 1997 - 1998
- Recognition
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In the News
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NOV 14, 2019 Duke Research Blog
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Awards & Honors
- Thomas Davis Research Award. American Lung Association of North Carolina. 2003
- Investigator in Pathogenesis of Infectious Diseases. Burroughs Wellcome Fund. 2002
- Burroughs Wellcome Career Award. Burroughs Wellcome. 1997
- Damon Runyon-Walter Winchell Postdoctoral Research Fellow. Damon Runyon-Walter Winchell Foundation. 1994
- Expertise
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Subject Headings
- Bacteria
- Bacterial Outer Membrane Proteins
- Bacterial Proteins
- Bacterial Toxins
- Bacteriophage T4
- Biofilms
- Biological Transport
- Cell Fractionation
- Cell Membrane
- Cystic Fibrosis
- Enterotoxigenic Escherichia coli
- Epithelial Cells
- Epithelium
- Escherichia coli
- Escherichia coli Proteins
- Exosomes
- Fimbriae, Bacterial
- Gram-Negative Bacteria
- Gram-Negative Bacterial Infections
- HT29 Cells
- Lipopolysaccharides
- Lipoproteins
- Macrophages
- Membrane Fusion
- Membrane Microdomains
- Membrane Proteins
- Membrane Transport Proteins
- Microbial Interactions
- Microbial Viability
- Molecular Chaperones
- Muser Mentor
- Periplasm
- Protein Sorting Signals
- Protein Transport
- Pseudomonas Infections
- Pseudomonas aeruginosa
- Secretory Vesicles
- Sigma Factor
- Transport Vesicles
- Vesicular Transport Proteins
- Virulence
- Research
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Selected Grants
- Cell and Molecular Biology Training Program awarded by National Institutes of Health 2021 - 2026
- Genetic and Genomics Training Grant awarded by National Institutes of Health 2020 - 2025
- IRGM proteins as regulators of inflammation awarded by National Institutes of Health 2020 - 2025
- Convergence RAISE: Harnessing Extracellular Vesicle Mediated Interkingdom Communication awarded by National Science Foundation 2019 - 2023
- GDEP STEM Outreach Supplement Award: Pay It Forward awarded by Burroughs Wellcome Fund 2021 - 2022
- Enteroendocrine cells sense gut bacteria and activate a gut-brain pathway awarded by National Institutes of Health 2021
- Characterizing the association of RNA with bacterial membrane vesicles and their delivery to host cells awarded by Burroughs Wellcome Fund 2019 - 2021
- Structural and Mechanistic Characterization of MraY Catalysis and Inhibition awarded by National Institutes of Health 2017 - 2020
- Genetics Training Grant awarded by National Institutes of Health 1979 - 2020
- Organization and Function of Cellular Structure awarded by National Institutes of Health 1975 - 2020
- Triggers and Consequences of Bacterial Envelope Stress awarded by National Institutes of Health 2012 - 2017
- Regulation of Host Defense by IRG Proteins awarded by National Institutes of Health 2004 - 2016
- Enterotoxin targeting and delivery mechanisms awarded by National Institutes of Health 2006 - 2012
- Duke PREP: Minority Recruitment into Biomedical Sciences awarded by National Institutes of Health 2003 - 2008
- Heat-Labile Enterotoxin Secretion awarded by National Institutes of Health 2005 - 2008
- Detection of in vivo ETEC vesicle production awarded by National Institutes of Health 2003 - 2006
- Production and function of E Coli Vesicles awarded by National Institutes of Health 2002 - 2004
- Publications & Artistic Works
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Selected Publications
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Academic Articles
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McMillan, Hannah M., and Meta J. Kuehn. “Proteomic Profiling Reveals Distinct Bacterial Extracellular Vesicle Subpopulations with Possibly Unique Functionality.” Appl Environ Microbiol, December 19, 2022, e0168622. https://doi.org/10.1128/aem.01686-22.Full Text Link to Item
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McMillan, Hannah M., and Meta J. Kuehn. “The extracellular vesicle generation paradox: a bacterial point of view.” Embo J 40, no. 21 (November 2, 2021): e108174. https://doi.org/10.15252/embj.2021108174.Full Text Link to Item
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McMillan, Hannah M., Nicholas Rogers, Austin Wadle, Heileen Hsu-Kim, Mark R. Wiesner, Meta J. Kuehn, and Christine Ogilvie Hendren. “Microbial vesicle-mediated communication: convergence to understand interactions within and between domains of life.” Environ Sci Process Impacts 23, no. 5 (May 26, 2021): 664–77. https://doi.org/10.1039/d1em00022e.Full Text Link to Item
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McMillan, Hannah M., Sophia G. Zebell, Jean B. Ristaino, Xinnian Dong, and Meta J. Kuehn. “Protective plant immune responses are elicited by bacterial outer membrane vesicles.” Cell Rep 34, no. 3 (January 19, 2021): 108645. https://doi.org/10.1016/j.celrep.2020.108645.Full Text Open Access Copy Link to Item
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Orench-Rivera, Nichole, and Meta J. Kuehn. “Differential Packaging Into Outer Membrane Vesicles Upon Oxidative Stress Reveals a General Mechanism for Cargo Selectivity.” Front Microbiol 12 (2021): 561863. https://doi.org/10.3389/fmicb.2021.561863.Full Text Link to Item
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Finethy, Ryan, Jacob Dockterman, Miriam Kutsch, Nichole Orench-Rivera, Graham D. Wallace, Anthony S. Piro, Sarah Luoma, et al. “Dynamin-related Irgm proteins modulate LPS-induced caspase-11 activation and septic shock.” Embo Rep 21, no. 11 (November 5, 2020): e50830. https://doi.org/10.15252/embr.202050830.Full Text Link to Item
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Rodriguez, Blanca V., and Meta J. Kuehn. “Staphylococcus aureus secretes immunomodulatory RNA and DNA via membrane vesicles.” Sci Rep 10, no. 1 (October 26, 2020): 18293. https://doi.org/10.1038/s41598-020-75108-3.Full Text Link to Item
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Zingl, Franz G., Paul Kohl, Fatih Cakar, Deborah R. Leitner, Fabian Mitterer, Katherine E. Bonnington, Gerald N. Rechberger, et al. “Outer Membrane Vesiculation Facilitates Surface Exchange and In Vivo Adaptation of Vibrio cholerae.” Cell Host Microbe 27, no. 2 (February 12, 2020): 225-237.e8. https://doi.org/10.1016/j.chom.2019.12.002.Full Text Open Access Copy Link to Item
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Finethy, Ryan, Jacob Dockterman, Miriam Kutsch, Nichole Orench-Rivera, Graham Wallace, Anthony Piro, Sarah Luoma, et al. “Dynamin-related Irgm proteins modulate LPS-induced caspase-4 activation and septic shock,” 2020. https://doi.org/10.1101/2020.03.18.997460.Full Text Open Access Copy
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Esoda, Caitlin N., and Meta J. Kuehn. “Pseudomonas aeruginosa Leucine Aminopeptidase Influences Early Biofilm Composition and Structure via Vesicle-Associated Antibiofilm Activity.” Mbio 10, no. 6 (November 19, 2019). https://doi.org/10.1128/mBio.02548-19.Full Text Link to Item
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Finethy, Ryan, Sarah Luoma, Nichole Orench-Rivera, Eric M. Feeley, Arun K. Haldar, Masahiro Yamamoto, Thirumala-Devi Kanneganti, Meta J. Kuehn, and Jörn Coers. “Inflammasome Activation by Bacterial Outer Membrane Vesicles Requires Guanylate Binding Proteins.” Mbio 8, no. 5 (October 3, 2017). https://doi.org/10.1128/mBio.01188-17.Full Text Open Access Copy Link to Item
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Bonnington, Katherine E., and Meta J. Kuehn. “Breaking the bilayer: OMV formation during environmental transitions.” Microb Cell 4, no. 2 (February 3, 2017): 64–66. https://doi.org/10.15698/mic2017.02.558.Full Text Open Access Copy Link to Item
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Orench-Rivera, Nichole, and Meta J. Kuehn. “Environmentally controlled bacterial vesicle-mediated export.” Cell Microbiol 18, no. 11 (November 2016): 1525–36. https://doi.org/10.1111/cmi.12676.Full Text Open Access Copy Link to Item
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Bonnington, Katherine E., and Meta J. Kuehn. “Outer Membrane Vesicle Production Facilitates LPS Remodeling and Outer Membrane Maintenance in Salmonella during Environmental Transitions.” Mbio 7, no. 5 (October 18, 2016). https://doi.org/10.1128/mBio.01532-16.Full Text Open Access Copy Link to Item
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Vogel, Robert, Frank A. W. Coumans, Raluca G. Maltesen, Anita N. Böing, Katherine E. Bonnington, Marike L. Broekman, Murray F. Broom, et al. “A standardized method to determine the concentration of extracellular vesicles using tunable resistive pulse sensing.” J Extracell Vesicles 5 (2016): 31242. https://doi.org/10.3402/jev.v5.31242.Full Text Open Access Copy Link to Item
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Schwechheimer, Carmen, and Meta J. Kuehn. “Outer-membrane vesicles from Gram-negative bacteria: biogenesis and functions.” Nat Rev Microbiol 13, no. 10 (October 2015): 605–19. https://doi.org/10.1038/nrmicro3525.Full Text Link to Item
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Schwechheimer, Carmen, Daniel L. Rodriguez, and Meta J. Kuehn. “NlpI-mediated modulation of outer membrane vesicle production through peptidoglycan dynamics in Escherichia coli.” Microbiologyopen 4, no. 3 (June 2015): 375–89. https://doi.org/10.1002/mbo3.244.Full Text Open Access Copy Link to Item
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Kulp, Adam J., Bo Sun, Teresa Ai, Andrew J. Manning, Nichole Orench-Rivera, Amy K. Schmid, and Meta J. Kuehn. “Genome-Wide Assessment of Outer Membrane Vesicle Production in Escherichia coli.” Plos One 10, no. 9 (2015): e0139200. https://doi.org/10.1371/journal.pone.0139200.Full Text Open Access Copy Link to Item
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Schwechheimer, Carmen, Adam Kulp, and Meta J. Kuehn. “Modulation of bacterial outer membrane vesicle production by envelope structure and content.” Bmc Microbiol 14 (December 21, 2014): 324. https://doi.org/10.1186/s12866-014-0324-1.Full Text Open Access Copy Link to Item
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Bonnington, K. E., and M. J. Kuehn. “Protein selection and export via outer membrane vesicles.” Biochim Biophys Acta 1843, no. 8 (August 2014): 1612–19. https://doi.org/10.1016/j.bbamcr.2013.12.011.Full Text Link to Item
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Chutkan, H., I. A. MacDonald, A. J. Manning, and M. J. Kuehn. “Quantitative and qualitative preparations of bacterial outer membrane vesicles.” Methods in Molecular Biology 966 (December 1, 2013): 259–72. https://doi.org/10.1007/978-1-62703-245-2-16.Full Text Open Access Copy
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Schwechheimer, Carmen, and Meta J. Kuehn. “Synthetic effect between envelope stress and lack of outer membrane vesicle production in Escherichia coli.” J Bacteriol 195, no. 18 (September 2013): 4161–73. https://doi.org/10.1128/JB.02192-12.Full Text Link to Item
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Macdonald, Ian A., and Meta J. Kuehn. “Stress-induced outer membrane vesicle production by Pseudomonas aeruginosa.” J Bacteriol 195, no. 13 (July 2013): 2971–81. https://doi.org/10.1128/JB.02267-12.Full Text Open Access Copy Link to Item
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Schwechheimer, Carmen, Claretta J. Sullivan, and Meta J. Kuehn. “Envelope control of outer membrane vesicle production in Gram-negative bacteria.” Biochemistry 52, no. 18 (May 7, 2013): 3031–40. https://doi.org/10.1021/bi400164t.Full Text Link to Item
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Manning, Andrew J., and Meta J. Kuehn. “Functional advantages conferred by extracellular prokaryotic membrane vesicles.” J Mol Microbiol Biotechnol 23, no. 1–2 (2013): 131–41. https://doi.org/10.1159/000346548.Full Text Link to Item
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Chutkan, Halima, Ian Macdonald, Andrew Manning, and Meta J. Kuehn. “Quantitative and qualitative preparations of bacterial outer membrane vesicles.” Methods Mol Biol 966 (2013): 259–72. https://doi.org/10.1007/978-1-62703-245-2_16.Full Text Link to Item
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Kuehn, Meta J. “Secreted bacterial vesicles as good samaritans.” Cell Host Microbe 12, no. 4 (October 18, 2012): 392–93. https://doi.org/10.1016/j.chom.2012.10.005.Full Text Link to Item
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MacDonald, Ian A., and Meta J. Kuehn. “Offense and defense: microbial membrane vesicles play both ways.” Res Microbiol 163, no. 9–10 (2012): 607–18. https://doi.org/10.1016/j.resmic.2012.10.020.Full Text Link to Item
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Tan, Cheemeng, Robert Phillip Smith, Jaydeep K. Srimani, Katherine A. Riccione, Sameer Prasada, Meta Kuehn, and Lingchong You. “The inoculum effect and band-pass bacterial response to periodic antibiotic treatment.” Mol Syst Biol 8 (2012): 617. https://doi.org/10.1038/msb.2012.49.Full Text Open Access Copy Link to Item
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Manning, Andrew J., and Meta J. Kuehn. “Contribution of bacterial outer membrane vesicles to innate bacterial defense.” Bmc Microbiol 11 (December 1, 2011): 258. https://doi.org/10.1186/1471-2180-11-258.Full Text Open Access Copy Link to Item
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Kulp, Adam, and Meta J. Kuehn. “Recognition of β-strand motifs by RseB is required for σ(E) activity in Escherichia coli.” J Bacteriol 193, no. 22 (November 2011): 6179–86. https://doi.org/10.1128/JB.05657-11.Full Text Link to Item
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Sharpe, Samantha W., Meta J. Kuehn, and Kevin M. Mason. “Elicitation of epithelial cell-derived immune effectors by outer membrane vesicles of nontypeable Haemophilus influenzae.” Infect Immun 79, no. 11 (November 2011): 4361–69. https://doi.org/10.1128/IAI.05332-11.Full Text Link to Item
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Chutkan, Halima, and Meta J. Kuehn. “Context-dependent activation kinetics elicited by soluble versus outer membrane vesicle-associated heat-labile enterotoxin.” Infect Immun 79, no. 9 (September 2011): 3760–69. https://doi.org/10.1128/IAI.05336-11.Full Text Link to Item
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Muralinath, Maneesha, Meta J. Kuehn, Kenneth L. Roland, and Roy Curtiss. “Immunization with Salmonella enterica serovar Typhimurium-derived outer membrane vesicles delivering the pneumococcal protein PspA confers protection against challenge with Streptococcus pneumoniae.” Infect Immun 79, no. 2 (February 2011): 887–94. https://doi.org/10.1128/IAI.00950-10.Full Text Link to Item
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Ellis, Terri N., Sara A. Leiman, and Meta J. Kuehn. “Naturally produced outer membrane vesicles from Pseudomonas aeruginosa elicit a potent innate immune response via combined sensing of both lipopolysaccharide and protein components.” Infect Immun 78, no. 9 (September 2010): 3822–31. https://doi.org/10.1128/IAI.00433-10.Full Text Link to Item
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Mudrak, Benjamin, and Meta J. Kuehn. “Heat-labile enterotoxin: beyond G(m1) binding.” Toxins (Basel) 2, no. 6 (June 2010): 1445–70. https://doi.org/10.3390/toxins2061445.Full Text Open Access Copy Link to Item
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Mudrak, Benjamin, and Meta J. Kuehn. “Specificity of the type II secretion systems of enterotoxigenic Escherichia coli and Vibrio cholerae for heat-labile enterotoxin and cholera toxin.” J Bacteriol 192, no. 7 (April 2010): 1902–11. https://doi.org/10.1128/JB.01542-09.Full Text Link to Item
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Ellis, Terri N., and Meta J. Kuehn. “Virulence and immunomodulatory roles of bacterial outer membrane vesicles.” Microbiol Mol Biol Rev 74, no. 1 (March 2010): 81–94. https://doi.org/10.1128/MMBR.00031-09.Full Text Link to Item
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Kulp, Adam, and Meta J. Kuehn. “Biological functions and biogenesis of secreted bacterial outer membrane vesicles.” Annu Rev Microbiol 64 (2010): 163–84. https://doi.org/10.1146/annurev.micro.091208.073413.Full Text Link to Item
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Mudrak, Benjamin, Daniel L. Rodriguez, and Meta J. Kuehn. “Residues of heat-labile enterotoxin involved in bacterial cell surface binding.” J Bacteriol 191, no. 9 (May 2009): 2917–25. https://doi.org/10.1128/JB.01622-08.Full Text Link to Item
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Bauman, Susanne J., and Meta J. Kuehn. “Pseudomonas aeruginosa vesicles associate with and are internalized by human lung epithelial cells.” Bmc Microbiol 9 (February 3, 2009): 26. https://doi.org/10.1186/1471-2180-9-26.Full Text Open Access Copy Link to Item
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McBroom, Amanda J., and Meta J. Kuehn. “Release of outer membrane vesicles by Gram-negative bacteria is a novel envelope stress response.” Mol Microbiol 63, no. 2 (January 2007): 545–58. https://doi.org/10.1111/j.1365-2958.2006.05522.x.Full Text Open Access Copy Link to Item
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Bauman, Susanne J., and Meta J. Kuehn. “Purification of outer membrane vesicles from Pseudomonas aeruginosa and their activation of an IL-8 response.” Microbes Infect 8, no. 9–10 (August 2006): 2400–2408. https://doi.org/10.1016/j.micinf.2006.05.001.Full Text Link to Item
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McBroom, Amanda J., Alexandra P. Johnson, Sreekanth Vemulapalli, and Meta J. Kuehn. “Outer membrane vesicle production by Escherichia coli is independent of membrane instability.” J Bacteriol 188, no. 15 (August 2006): 5385–92. https://doi.org/10.1128/JB.00498-06.Full Text Link to Item
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Kuehn, Meta J. “Genetically engineered probiotic competition.” Gastroenterology 130, no. 6 (May 2006): 1915–16. https://doi.org/10.1053/j.gastro.2006.03.031.Full Text Link to Item
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Kuehn, Meta J., and Nicole C. Kesty. “Bacterial outer membrane vesicles and the host-pathogen interaction.” Genes Dev 19, no. 22 (November 15, 2005): 2645–55. https://doi.org/10.1101/gad.1299905.Full Text Link to Item
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McBroom, Amanda J., and Meta J. Kuehn. “Outer Membrane Vesicles.” Ecosal Plus 1, no. 2 (November 2005). https://doi.org/10.1128/ecosal.2.2.4.Full Text Link to Item
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Kesty, Nicole C., Kevin M. Mason, Mary Reedy, Sara E. Miller, and Meta J. Kuehn. “Enterotoxigenic Escherichia coli vesicles target toxin delivery into mammalian cells.” Embo J 23, no. 23 (November 24, 2004): 4538–49. https://doi.org/10.1038/sj.emboj.7600471.Full Text Link to Item
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Horstman, Amanda L., Susanne J. Bauman, and Meta J. Kuehn. “Lipopolysaccharide 3-deoxy-D-manno-octulosonic acid (Kdo) core determines bacterial association of secreted toxins.” J Biol Chem 279, no. 9 (February 27, 2004): 8070–75. https://doi.org/10.1074/jbc.M308633200.Full Text Link to Item
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Kesty, Nicole C., and Meta J. Kuehn. “Incorporation of heterologous outer membrane and periplasmic proteins into Escherichia coli outer membrane vesicles.” J Biol Chem 279, no. 3 (January 16, 2004): 2069–76. https://doi.org/10.1074/jbc.M307628200.Full Text Link to Item
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Kuehn, M. “The E. coli BaeSR two-component regulatory system.” Trends in Microbiology 10, no. 12 (December 1, 2002): 553. https://doi.org/10.1016/s0966-842x(02)02484-8.Full Text
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Kuehn, M. J. “Bacterial density dictates virulence in cholera.” Trends in Microbiology 10, no. 10 (October 1, 2002): 449. https://doi.org/10.1016/s0966-842x(02)02455-1.Full Text
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Horstman, Amanda L., and Meta J. Kuehn. “Bacterial surface association of heat-labile enterotoxin through lipopolysaccharide after secretion via the general secretory pathway.” J Biol Chem 277, no. 36 (September 6, 2002): 32538–45. https://doi.org/10.1074/jbc.M203740200.Full Text Link to Item
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Kuehn, M. J. “FRET probes toxin activity in situ.” Trends in Microbiology 10, no. 8 (August 1, 2002): 355. https://doi.org/10.1016/S0966-842X(02)02414-9.Full Text
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Kuehn, M. J. “Surrogate host succumbs to virulent Pseudomonas.” Trends in Microbiology 10, no. 5 (May 1, 2002): 215. https://doi.org/10.1016/s0966-842x(02)02348-x.Full Text
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Kuehn, Meta J. “Polarized secretion.” Trends Microbiol 10, no. 3 (March 2002): 116. https://doi.org/10.1016/s0966-842x(02)02312-0.Full Text Link to Item
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Kuehn, M. J. “Engineering a biosensor from a bacterial periplasmic protein.” Trends in Microbiology 9, no. 11 (November 1, 2001): 527. https://doi.org/10.1016/s0966-842x(01)02223-5.Full Text
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Kuehn, M. J. “Bacterial-host-cell tethers.” Trends Microbiol 9, no. 7 (July 2001): 310. https://doi.org/10.1016/s0966-842x(01)02109-6.Full Text Link to Item
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Kuehn, M. J. “Bacterial cave dwellers.” Trends Microbiol 8, no. 10 (October 2000): 450–51. https://doi.org/10.1016/s0966-842x(00)01867-9.Full Text Link to Item
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Kuehn, M. “Definitive typing of LPS core structures.” Trends in Microbiology 8, no. 5 (May 1, 2000): 212. https://doi.org/10.1016/s0966-842x(00)01766-2.Full Text
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Horstman, A. L., and M. J. Kuehn. “Enterotoxigenic Escherichia coli secretes active heat-labile enterotoxin via outer membrane vesicles.” J Biol Chem 275, no. 17 (April 28, 2000): 12489–96. https://doi.org/10.1074/jbc.275.17.12489.Full Text Link to Item
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Kuehn, M. “Lighting the path to virulence.” Trends in Microbiology 8, no. 1 (January 1, 2000): 16. https://doi.org/10.1016/s0966-842x(99)01659-5.Full Text
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Kuehn, M. “Foreign travel.” Trends Microbiol 7, no. 3 (March 1999): 102. https://doi.org/10.1016/s0966-842x(99)01469-9.Full Text Link to Item
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Kuehn, M. “Expressed by stress.” Trends in Microbiology 7, no. 6 (January 1, 1999): 231. https://doi.org/10.1016/s0966-842x(99)01518-8.Full Text
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Kuehn, M. “A divisive role for lipoproteins.” Trends in Microbiology 7, no. 10 (January 1, 1999): 400. https://doi.org/10.1016/s0966-842x(99)01587-5.Full Text
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Kuehn, M. J., J. M. Herrmann, and R. Schekman. “COPII-cargo interactions direct protein sorting into ER-derived transport vesicles.” Nature 391, no. 6663 (January 8, 1998): 187–90. https://doi.org/10.1038/34438.Full Text Link to Item
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Kuehn, M. “New transporter family.” Trends in Microbiology 6, no. 9 (January 1, 1998): 351. https://doi.org/10.1016/S0966-842X(98)01335-3.Full Text
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Schekman, R., S. Hamamoto, M. Kuehn, K. Matsuoka, T. Yeung, and L. Orci. “Cargo selection and vesicle budding.” Faseb Journal 11, no. 9 (December 1, 1997).
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Kuehn, M. J., and R. Schekman. “COPII and secretory cargo capture into transport vesicles.” Curr Opin Cell Biol 9, no. 4 (August 1997): 477–83. https://doi.org/10.1016/s0955-0674(97)80022-1.Full Text Link to Item
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Kuehn, M. J. “Establishing communication via gram-negative bacterial pili.” Trends Microbiol 5, no. 4 (April 1997): 130–32. https://doi.org/10.1016/S0966-842X(96)30045-0.Full Text Link to Item
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Kuehn, M. “Leaping into the outer membrane.” Trends in Microbiology 5, no. 10 (January 1, 1997): 387. https://doi.org/10.1016/s0966-842x(97)89757-0.Full Text
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Kuehn, M. “Cryptococcus and calcineurin.” Trends in Microbiology 5, no. 8 (January 1, 1997): 307. https://doi.org/10.1016/s0966-842x(97)82230-5.Full Text
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Kuehn, M. J., R. Schekman, and P. O. Ljungdahl. “Amino acid permeases require COPII components and the ER resident membrane protein Shr3p for packaging into transport vesicles in vitro.” J Cell Biol 135, no. 3 (November 1996): 585–95. https://doi.org/10.1083/jcb.135.3.585.Full Text Open Access Copy Link to Item
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Schekman, R., C. Barlowe, S. Bednarek, J. Campbell, T. Doering, R. Duden, M. Kuehn, M. Rexach, T. Yeung, and L. Orci. “Coat proteins and selective protein packaging into transport vesicles.” Cold Spring Harb Symp Quant Biol 60 (1995): 11–21. https://doi.org/10.1101/sqb.1995.060.01.004.Full Text Link to Item
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Kuehn, M. J., F. Jacob-Dubuisson, K. Dodson, L. Slonim, R. Striker, and S. J. Hultgren. “Genetic, biochemical, and structural studies of biogenesis of adhesive pili in bacteria.” Methods Enzymol 236 (1994): 282–306. https://doi.org/10.1016/0076-6879(94)36022-7.Full Text Link to Item
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Kuehn, M. J., D. J. Ogg, J. Kihlberg, L. N. Slonim, K. Flemmer, T. Bergfors, and S. J. Hultgren. “Structural basis of pilus subunit recognition by the PapD chaperone.” Science 262, no. 5137 (November 19, 1993): 1234–41. https://doi.org/10.1126/science.7901913.Full Text Link to Item
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Jacob-Dubuisson, F., M. Kuehn, and S. J. Hultgren. “A novel secretion apparatus for the assembly of adhesive bacterial pili.” Trends Microbiol 1, no. 2 (May 1993): 50–55. https://doi.org/10.1016/0966-842x(93)90032-m.Full Text Link to Item
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Jones, C. H., F. Jacob-Dubuisson, K. Dodson, M. Kuehn, L. Slonim, R. Striker, and S. J. Hultgren. “Adhesin presentation in bacteria requires molecular chaperones and ushers.” Infect Immun 60, no. 11 (November 1992): 4445–51. https://doi.org/10.1128/iai.60.11.4445-4451.1992.Full Text Link to Item
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Kuehn, M., K. Lent, J. Haas, J. Hagenzieker, M. Cervin, and A. L. Smith. “Fimbriation of Pseudomonas cepacia.” Infect Immun 60, no. 5 (May 1992): 2002–7. https://doi.org/10.1128/iai.60.5.2002-2007.1992.Full Text Link to Item
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Holmgren, A., M. J. Kuehn, C. I. Brändén, and S. J. Hultgren. “Conserved immunoglobulin-like features in a family of periplasmic pilus chaperones in bacteria.” Embo J 11, no. 4 (April 1992): 1617–22. https://doi.org/10.1002/j.1460-2075.1992.tb05207.x.Full Text Link to Item
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Kuehn, M. J., J. Heuser, S. Normark, and S. J. Hultgren. “P pili in uropathogenic E. coli are composite fibres with distinct fibrillar adhesive tips.” Nature 356, no. 6366 (March 19, 1992): 252–55. https://doi.org/10.1038/356252a0.Full Text Link to Item
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Kuehn, M. J., S. Normark, and S. J. Hultgren. “Immunoglobulin-like PapD chaperone caps and uncaps interactive surfaces of nascently translocated pilus subunits.” Proc Natl Acad Sci U S A 88, no. 23 (December 1, 1991): 10586–90. https://doi.org/10.1073/pnas.88.23.10586.Full Text Link to Item
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Groot, R. de, D. O. Chaffin, M. Kuehn, and A. L. Smith. “Trimethoprim resistance in Haemophilus influenzae is due to altered dihydrofolate reductase(s).” Biochem J 274 ( Pt 3), no. Pt 3 (March 15, 1991): 657–62.Link to Item
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Chanyangam, M., A. L. Smith, S. L. Moseley, M. Kuehn, and P. Jenny. “Contribution of a 28-kilodalton membrane protein to the virulence of Haemophilus influenzae.” Infect Immun 59, no. 2 (February 1991): 600–608. https://doi.org/10.1128/iai.59.2.600-608.1991.Full Text Link to Item
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Conference Papers
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Mcmillan, H. M., S. G. Zebell, X. Dong, and M. J. Kuehn. “Bacterial vesicles: Double agents for plant defense.” In Molecular Plant Microbe Interactions, 32:34–35. AMER PHYTOPATHOLOGICAL SOC, 2019.Link to Item
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Orench-Rivera, Nichole, and Meta Kuehn. “OXIDATIVE STRESS-INDUCED DIFFERENTIAL PACKAGING OF PROTEINS IN ENTEROTOXIGENIC ESCHERICHIA COLIOUTER MEMBRANE VESICLES AND ITS IMPACT ON HOST-PATHOGEN INTERACTIONS.” In American Journal of Tropical Medicine and Hygiene, 99:143–143. AMER SOC TROP MED & HYGIENE, 2018.Link to Item
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Preprints
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McMillan, Hannah, Sophia Zebell, Jean Ristaino, Xinnian Dong, and Meta Kuehn. “Protective Plant Immune Responses are Elicited by Bacterial Outer Membrane Vesicles.” BioRxiv, 2020. https://doi.org/10.1101/2020.07.24.220160.Full Text
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Rodriguez, Blanca, and Meta Kuehn. “Staphylococcus aureus secretes immunomodulatory RNA and DNA via membrane vesicles.” BioRxiv, 2020. https://doi.org/10.1101/2020.09.23.310318.Full Text
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Esoda, Caitlin, and Meta Kuehn. “Pseudomonas aeruginosaleucine aminopeptidase influences early biofilm composition and structure via vesicle-associated anti-biofilm activity.” BioRxiv, 2019. https://doi.org/10.1101/784918.Full Text
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Esoda, Caitlin, and Meta Kuehn. “Vesicle-associatedPseudomonas aeruginosaleucine aminopeptidase modulates bacterial biofilms grown on host cellular substrates.” BioRxiv, 2019. https://doi.org/10.1101/587279.Full Text
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