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DNA sequence and genetic characterization of plasmid pFQ11 from Frankia alni strain CpI1.

Publication ,  Journal Article
Xu, X; Kong, R; de Bruijn, FJ; He, SY; Murry, MA; Newman, T; Wolk, CP
Published in: FEMS microbiology letters
January 2002

An 8551-bp plasmid, pFQ11, from Frankia alni strain CpI1 was sequenced. Its sequence was found to be very similar to that presented for pFQ31 from strain ArI3. Six potential protein-encoding open reading frames (ORFs) were identified, and transcriptional activity was shown within four of those regions of the plasmid by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. An earlier study reported that ORF E(F) of pFQ31, which is nearly identical to the 3' 45% of ORF1 of pFQ11, is significantly similar to RepF. We found no such similarity. ORF2 and ORF3 predict products that are similar to a repressor protein and a partition protein, respectively. We found inverted repeats within and covering the start codon of ORF3; palindromic sequences and direct repeats between ORF3 and ORF4; and 3' from ORF3, an AT-rich sequence that extensively overlaps the promoter region of a uvrB homolog in strain ArI3.

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Published In

FEMS microbiology letters

DOI

EISSN

1574-6968

ISSN

0378-1097

Publication Date

January 2002

Volume

207

Issue

1

Start / End Page

103 / 107

Related Subject Headings

  • Sequence Analysis, DNA
  • Reverse Transcriptase Polymerase Chain Reaction
  • Plasmids
  • Molecular Sequence Data
  • Microbiology
  • Base Sequence
  • Bacterial Proteins
  • Actinomycetales
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
 

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Xu, X., Kong, R., de Bruijn, F. J., He, S. Y., Murry, M. A., Newman, T., & Wolk, C. P. (2002). DNA sequence and genetic characterization of plasmid pFQ11 from Frankia alni strain CpI1. FEMS Microbiology Letters, 207(1), 103–107. https://doi.org/10.1111/j.1574-6968.2002.tb11036.x
Xu, Xudong, Renqiu Kong, Frans J. de Bruijn, Sheng Yang He, Marcia A. Murry, Thomas Newman, and C Peter Wolk. “DNA sequence and genetic characterization of plasmid pFQ11 from Frankia alni strain CpI1.FEMS Microbiology Letters 207, no. 1 (January 2002): 103–7. https://doi.org/10.1111/j.1574-6968.2002.tb11036.x.
Xu X, Kong R, de Bruijn FJ, He SY, Murry MA, Newman T, et al. DNA sequence and genetic characterization of plasmid pFQ11 from Frankia alni strain CpI1. FEMS microbiology letters. 2002 Jan;207(1):103–7.
Xu, Xudong, et al. “DNA sequence and genetic characterization of plasmid pFQ11 from Frankia alni strain CpI1.FEMS Microbiology Letters, vol. 207, no. 1, Jan. 2002, pp. 103–07. Epmc, doi:10.1111/j.1574-6968.2002.tb11036.x.
Xu X, Kong R, de Bruijn FJ, He SY, Murry MA, Newman T, Wolk CP. DNA sequence and genetic characterization of plasmid pFQ11 from Frankia alni strain CpI1. FEMS microbiology letters. 2002 Jan;207(1):103–107.
Journal cover image

Published In

FEMS microbiology letters

DOI

EISSN

1574-6968

ISSN

0378-1097

Publication Date

January 2002

Volume

207

Issue

1

Start / End Page

103 / 107

Related Subject Headings

  • Sequence Analysis, DNA
  • Reverse Transcriptase Polymerase Chain Reaction
  • Plasmids
  • Molecular Sequence Data
  • Microbiology
  • Base Sequence
  • Bacterial Proteins
  • Actinomycetales
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences