Conversion of compatible plant-pathogen interactions into incompatible interactions by expression of the Pseudomonas syringae pv. syringae 61 hrmA gene in transgenic tobacco plants.

Journal Article (Journal Article)

The hrmA gene from Pseudomonas syringae pv. syringae has previously been shown to confer avirulence on the virulent bacterium P. syringae pv. tabaci in all examined tobacco cultivars. We expressed this gene in tobacco plants under the control of the tobacco Delta0. 3 TobRB7 promoter, which is induced upon nematode infection in tobacco roots (Opperman et al. 1994, Science, 263, 221-223). A basal level of hrmA expression in leaves of transgenic plants activated the expression of pathogenesis-related genes, and the transgenic plants exhibited high levels of resistance to multiple pathogens: tobacco vein mottling virus, tobacco etch virus, black shank fungus Phytophthora parasitica, and wild fire bacterium Pseudomonas syringae pv. tabaci. However, the hrmA transgenic plants were not significantly more resistant to root-knot nematodes. Our results suggest a potential use of controlled low-level expression of bacterial avr genes, such as hrmA, in plants to generate broad-spectrum resistance to bacterial, fungal and viral pathogens.

Full Text

Duke Authors

Cited Authors

  • Shen, S; Li, Q; He, SY; Barker, KR; Li, D; Hunt, AG

Published Date

  • July 2000

Published In

Volume / Issue

  • 23 / 2

Start / End Page

  • 205 - 213

PubMed ID

  • 10929114

Electronic International Standard Serial Number (EISSN)

  • 1365-313X

International Standard Serial Number (ISSN)

  • 0960-7412

Digital Object Identifier (DOI)

  • 10.1046/j.1365-313x.2000.00772.x


  • eng