Role of the Hrp pilus in type III protein secretion in Pseudomonas syringae.

Journal Article (Journal Article)

Bacterial surface appendages called pili and needle-like filaments are associated with protein and/or DNA transfer to recipient plant, human, or bacterial cells during pathogenesis or conjugation. Although it has long been suspected that pili function as a conduit for protein or DNA transfer, direct evidence has been lacking. The Hrp pilus of Pseudomonas syringae is assembled by the type III secretion system. We used an in situ immunogold labeling procedure to visualize the extrusion of an effector protein, AvrPto, from the tip of the Hrp pilus, providing direct evidence that a bacterial pilus can function as a conduit for protein delivery.

Full Text

Duke Authors

Cited Authors

  • Jin, Q; He, SY

Published Date

  • December 2001

Published In

Volume / Issue

  • 294 / 5551

Start / End Page

  • 2556 - 2558

PubMed ID

  • 11752577

Pubmed Central ID

  • 11752577

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.1066397

Language

  • eng