Role of the Hrp pilus in type III protein secretion in Pseudomonas syringae.
Journal Article (Journal Article)
Bacterial surface appendages called pili and needle-like filaments are associated with protein and/or DNA transfer to recipient plant, human, or bacterial cells during pathogenesis or conjugation. Although it has long been suspected that pili function as a conduit for protein or DNA transfer, direct evidence has been lacking. The Hrp pilus of Pseudomonas syringae is assembled by the type III secretion system. We used an in situ immunogold labeling procedure to visualize the extrusion of an effector protein, AvrPto, from the tip of the Hrp pilus, providing direct evidence that a bacterial pilus can function as a conduit for protein delivery.
Full Text
Duke Authors
Cited Authors
- Jin, Q; He, SY
Published Date
- December 2001
Published In
Volume / Issue
- 294 / 5551
Start / End Page
- 2556 - 2558
PubMed ID
- 11752577
Pubmed Central ID
- 11752577
Electronic International Standard Serial Number (EISSN)
- 1095-9203
International Standard Serial Number (ISSN)
- 0036-8075
Digital Object Identifier (DOI)
- 10.1126/science.1066397
Language
- eng